Skip to main content

Main menu

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons

User menu

  • Subscribe
  • Log in

Search

  • Advanced search
The Journal of Immunology
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons
  • Subscribe
  • Log in
The Journal of Immunology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Follow The Journal of Immunology on Twitter
  • Follow The Journal of Immunology on RSS
Open Access

Antibody Binding to Cryptococcus neoformans Impairs Budding by Altering Capsular Mechanical Properties

Radames J. B. Cordero, Bruno Pontes, Susana Frases, Antonio S. Nakouzi, Leonardo Nimrichter, Marcio L. Rodrigues, Nathan B. Viana and Arturo Casadevall
J Immunol January 1, 2013, 190 (1) 317-323; DOI: https://doi.org/10.4049/jimmunol.1202324
Radames J. B. Cordero
*Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bruno Pontes
†Laboratório de Pinças Óticas, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-590 Brazil;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Susana Frases
‡Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-902 Brazil;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Antonio S. Nakouzi
*Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Leonardo Nimrichter
§Laboratório de Estudos Integrados em Bioquímica Microbiana, Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-902 Brazil;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marcio L. Rodrigues
§Laboratório de Estudos Integrados em Bioquímica Microbiana, Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-902 Brazil;
¶Fundação Oswaldo Cruz, Centro de Desenvolvimento Tecnológico em Saúde, Rio de Janeiro, 21040-360 Brazil; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nathan B. Viana
†Laboratório de Pinças Óticas, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-590 Brazil;
‖Instituto de Física, Universidade Federal do Rio de Janeiro Rio de Janeiro, 21941-909 Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Arturo Casadevall
*Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF + SI
  • PDF
Loading

Abstract

Abs to microbial capsules are critical for host defense against encapsulated pathogens, but very little is known about the effects of Ab binding on the capsule, apart from producing qualitative capsular reactions (“quellung” effects). A problem in studying Ab–capsule interactions is the lack of experimental methodology, given that capsules are fragile, highly hydrated structures. In this study, we pioneered the use of optical tweezers microscopy to study Ab–capsule interactions. Binding of protective mAbs to the capsule of the fungal pathogen Cryptococcus neoformans impaired yeast budding by trapping newly emerging buds inside the parental capsule. This effect is due to profound mAb-mediated changes in capsular mechanical properties, demonstrated by a concentration-dependent increase in capsule stiffness. This increase involved mAb-mediated cross-linking of capsular polysaccharide molecules. These results provide new insights into Ab-mediated immunity, while suggesting a new nonclassical mechanism of Ab function, which may apply to other encapsulated pathogens. Our findings add to the growing body of evidence that Abs have direct antimicrobial functions independent of other components of the immune system.

Footnotes

  • This work was supported by National Institutes of Health Grants AI033774, HL059842, and AI033142 to A.C. and R.J.B.C. R.J.B.C. was also supported in part by the Training Program in Cellular and Molecular Biology and Genetics Grant T32 GM007491. The Casadevall laboratory is part of and participates in the Center for AIDS Research at the Albert Einstein College of Medicine and Montefiore Medical Center, funded by the National Institutes of Health (AI51519). B.P. and N.B.V. received grants from Conselho Nacional de Desenvolvimento Tecnológico, Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, and Instituto Nacional de Ciência e Tecnologia de Fluidos Complexos. S.F. received grants from Conselho Nacional de Desenvolvimento Tecnológico. M.L.R. and L.N. received grants from Conselho Nacional de Desenvolvimento Tecnológico, Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Fundação de Amparo à Pesquisa do Estado de São Paulo, and Financiadora de Estudos e Projetos.

  • The online version of this article contains supplemental material.

  • Abbreviations used in this article:

    DLS
    dynamic light scattering
    GXM
    glucuronoxylomannan
    PS
    polysaccharide.

  • Received August 20, 2012.
  • Accepted November 1, 2012.
  • Copyright © 2012 by The American Association of Immunologists, Inc.

This article is distributed under The American Association of Immunologists, Inc., Reuse Terms and Conditions for Author Choice articles.

View Full Text
PreviousNext
Back to top

In this issue

The Journal of Immunology: 190 (1)
The Journal of Immunology
Vol. 190, Issue 1
1 Jan 2013
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Advertising (PDF)
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about The Journal of Immunology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Antibody Binding to Cryptococcus neoformans Impairs Budding by Altering Capsular Mechanical Properties
(Your Name) has forwarded a page to you from The Journal of Immunology
(Your Name) thought you would like to see this page from the The Journal of Immunology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Antibody Binding to Cryptococcus neoformans Impairs Budding by Altering Capsular Mechanical Properties
Radames J. B. Cordero, Bruno Pontes, Susana Frases, Antonio S. Nakouzi, Leonardo Nimrichter, Marcio L. Rodrigues, Nathan B. Viana, Arturo Casadevall
The Journal of Immunology January 1, 2013, 190 (1) 317-323; DOI: 10.4049/jimmunol.1202324

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Antibody Binding to Cryptococcus neoformans Impairs Budding by Altering Capsular Mechanical Properties
Radames J. B. Cordero, Bruno Pontes, Susana Frases, Antonio S. Nakouzi, Leonardo Nimrichter, Marcio L. Rodrigues, Nathan B. Viana, Arturo Casadevall
The Journal of Immunology January 1, 2013, 190 (1) 317-323; DOI: 10.4049/jimmunol.1202324
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like

Jump to section

  • Article
    • Abstract
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Disclosures
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF + SI
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Intestinal IL-17R Signaling Controls Secretory IgA and Oxidase Balance in Citrobacter rodentium Infection
  • Acidic Calcium-Independent Phospholipase A2 Regulates Eosinophil-Mediated Pathology during Filarial Manifestation of Tropical Pulmonary Eosinophilia
  • Transcriptional Diversity and Niche-Specific Distribution of Leukocyte Populations during Staphylococcus aureus Craniotomy-Associated Biofilm Infection
Show more INFECTIOUS DISEASE AND HOST RESPONSE

Similar Articles

Navigate

  • Home
  • Current Issue
  • Next in The JI
  • Archive
  • Brief Reviews
  • Pillars of Immunology
  • Translating Immunology

For Authors

  • Submit a Manuscript
  • Instructions for Authors
  • About the Journal
  • Journal Policies
  • Editors

General Information

  • Advertisers
  • Subscribers
  • Rights and Permissions
  • Accessibility Statement
  • Public Access
  • Privacy Policy
  • Disclaimer

Journal Services

  • Email Alerts
  • RSS Feeds
  • ImmunoCasts
  • Twitter

Copyright © 2021 by The American Association of Immunologists, Inc.

Print ISSN 0022-1767        Online ISSN 1550-6606