Gee, K., J. B. Angel, S. Mishra, M. A. Blahoianu, and A. Kumar. 2007. IL-10 regulation by HIV-Tat in primary human monocytic cells: involvement of calmodulin/calmodulin-dependent protein kinase-activated p38 MAPK and Sp-1 and CREB-1 transcription factors. J. Immunol. 178: 798–807.
In Fig. 2A, the bottom of the three flow cytometry histograms is incorrect because it is not data from primary monocytes. The data included in the top two histograms demonstrates that Tat treatment of human monocytes induces calcium influx. The bottom panel demonstrating calcium influx in response to ionophore was included solely as a positive control for the calcium flux assay. It is well documented that enhanced calcium influx is observed upon ionophore treatment. Removal of this data from the revised figure does not require any changes to the text of the Results. The corrected figure is shown below with a revised figure legend.
HIV-Tat-induced calcium influx plays a role in Tat-induced IL-10 expression. A, HIV Tat induces calcium influx in human monocytic cells. Monocytes (0.5 × 106 cells/ml) were loaded with Fluo-3/AM and stimulated with HIV-Tat (50 ng/ml) for 0–12 min. The resulting calcium influx was measured by flow cytometric analysis. Top panel, Unstimulated cells. Bottom panel, Stimulation with HIV-Tat (50 ng/ml) followed by the addition of EGTA. B, Tat-induced IL-10 production is regulated by Ca2+ influx. Human monocytes (0.5 × 106 cells/ml) were pretreated with EGTA (1–5 μM) for 2 h before stimulation with HIV-Tat (50 ng/ml) for an additional 24 h. IL-10 production was measured by ELISA. The results shown are mean ± SD of three different experiments.
- Copyright © 2012 by The American Association of Immunologists, Inc.
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