Correction: TREM2 and β-catenin regulate bone homeostasis by controlling the rate of osteoclastogenesis

Karel Otero; Masahiro Shinohara; Haibo Zhao; Marina Cella; Susan Gilfillan; Angela Colucci; Roberta Faccio; F. Patrick Ross; Steve L. Teitelbaum; Hiroshi Takayanagi; Marco Colonna

doi:10.4049/jimmunol.1290014

Otero, K., M. Shinohara, H. Zhao, M. Cella, S. Gilfillan, A. Colucci, R. Faccio, F. P. Ross, S. L. Teitelbaum, H. Takayanagi, and M. Colonna. 2012. TREM2 and β-catenin regulate bone homeostasis by controlling the rate of osteoclastogenesis. J. Immunol. 188: 2612–2621.

The original article contains errors in the legend for Fig. 2 and in Fig. 4C and 4F. In the legend for Fig. 2B, the first range for the nuclei in each osteoclast was enumerated incorrectly and should have been indicated as 3–5, not 3–10. In Fig. 4, the panels corresponding to osteoclasts generated from βcat^Δ/Δ mice after 4 days of culture (Fig. 4C) and 7 days of culture (Fig. 4F) are duplicated. The corrected images are shown here, along with the rest of Fig. 4. The first range for the nuclei in each osteoclast was enumerated incorrectly in the legend for Fig. 4D and should have been indicated as 3–5, not 3–10. The corrected legend is shown below. The original description of the results does not change. The authors deeply regret this oversight.

FIGURE 4.

β-catenin–deficient OcP proliferate less to M-CSF and exhibit accelerated osteoclastogenesis. (A) Immunoblot analysis of β-catenin and actin (loading control) in OcP total protein lysates from control LysM-Cre^+/+β-catenin^+/+ (βcat^+/+) and LysM-Cre^+/+β-catenin^fl/fl (βcat^Δ/Δ) mice. (B) Defect of M-CSF–induced proliferation in β-catenin–deficient OcP. Proliferation of βcat^+/+ and βcat^Δ/Δ OcP was measured 12 h after incubation with BrdU in the presence of 100 ng/ml M-CSF. (C and D) OcP from βcat^+/+ and βcat^Δ/Δ mice were cultured with 10 ng/mlM-CSF and 100 ng/ml RANKL to generate multinuclear OC. Development of OC was monitored at different time points by TRAP staining. (C) Representative images of TRAP-stained cultures. Original magnification ×200. (D) The number of TRAP-positive cells containing three or more nuclei was scored (total). Additionally, the nuclei in each OC was enumerated as follows: 3–5, 6–10, and >10 nuclei per TRAP-positive cell. (E) Effect of β-catenin deficiency on the expression of the osteoclastic differentiation markers Nfatc1 (encoding NFATc1), Acp5 (encoding TRAP), Ctsk (encoding cathepsin K), and Calcr (encoding calcitonin receptor) by quantitative PCR. (F and G) OC differentiation induced on bone slices. (F) After 4 and 7 d differentiation, cultures were fixed and stained with phalloidin-FITC to visualize the OC actin rings. Original magnification ×200. (G) Bone resorption pits were revealed by lectin staining of the bone slices (brown reaction product). Original magnification ×200. Results are representative of at least three separate experiments. *p < 0.05, **p < 0.01.