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In Results, under the subhead titled SGLT-1 activation blocks NF-κB nuclear translocation induced by LPS and CpG-ODN, the corrected text in the fourth, fifth, and six sentences of the paragraph should read as follows: “In HT-29, LCC-18, and STC-1 cells stimulated with LPS or CpG-ODN, we observed activation of NF-κB, i.e., translocation to the nucleus, whereas this activation was not detected in cells pretreated with d-glucose (Fig. 6⇓A) or 3-OMG (data not shown). NF-κB in the cytoplasm is complexed to members of the IκB family of inhibitory proteins. Western blot analysis revealed degradation of IκB when NF-κB translocates to the nucleus upon LPS or CpG-ODN stimulation; degradation of IκB in stimulated cells was inhibited by d-glucose (Fig. 6⇓B) or 3-OMG (data not shown) pretreatment.”
In addition, changes have been made in Fig. 6⇓ concerning the Western blots in B for LCC-18 and STC-1 and minor changes have also been made to the figure legend. Both the revised Fig. 6⇓ and the revised legend are shown below.
A, Western blot analysis for NF-κB in IEC nuclear extracts. HT29, LCC-18, and STC-1 cells were stimulated with LPS or CpG-ODN with or without d-glucose pretreatment. Nuclear extracts were analyzed by Western blotting with anti-NF-κB p65 Ab. B, Western blot analysis for IκB in IEC extracts. HT29, LCC-18, and STC-1 cells were stimulated with LPS or CpG-ODN with or without d-glucose pretreatment. Total protein extracts were analyzed by Western blotting with anti-IκB Ab. C, Involvement of Akt signaling pathway. HT29 protein extracts, following LPS and/or glucose treatment, were analyzed by Western blotting with anti-phospho-Akt and anti-Akt Abs (C1). In addition, cells were stimulated with LPS, with or without d-glucose pretreatment, in the presence or absence of Akt inhibitor LY294002. Nuclear extracts were analyzed by Western blotting with anti-NF-κB p65 Ab (C2). Untr, Untreated.
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