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Innate BALB/c Enteric Epithelial Responses to Trichinella spiralis: Inducible Expression of a Novel Goblet Cell Lectin, Intelectin-2, and Its Natural Deletion in C57BL/10 Mice

Alan D. Pemberton, Pamela A. Knight, John Gamble, William H. Colledge, Jin-Kyu Lee, Michael Pierce and Hugh R. P. Miller
J Immunol August 1, 2004, 173 (3) 1894-1901; DOI: https://doi.org/10.4049/jimmunol.173.3.1894
Alan D. Pemberton
*Division of Veterinary Clinical Studies, University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian, United Kingdom;
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Pamela A. Knight
*Division of Veterinary Clinical Studies, University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian, United Kingdom;
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John Gamble
†Department of Physiology, University of Cambridge, Cambridge, United Kingdom; and
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William H. Colledge
†Department of Physiology, University of Cambridge, Cambridge, United Kingdom; and
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Jin-Kyu Lee
‡Department of Biochemistry, University of Georgia, Athens, GA 30602-7229
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Michael Pierce
‡Department of Biochemistry, University of Georgia, Athens, GA 30602-7229
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Hugh R. P. Miller
*Division of Veterinary Clinical Studies, University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian, United Kingdom;
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Abstract

Infection of mice with the nematode parasite Trichinella spiralis induces changes in the proteome of the jejunal epithelium, including substantial up-regulation of a novel variant of interlectin. In this study we sequence this novel lectin, termed intelectin-2, and compare expression levels during T. spiralis infection of resistant (BALB/c) with susceptible (C57BL/10) mouse strains. Intelectin-2 was cloned and sequenced from BALB/c mRNA extracted on day 14 of infection, and was found to have 91% amino acid identity with intelectin (within our study termed intelectin-1). Intelectin-2 transcripts were up-regulated early (day 3) during infection with T. spiralis in BALB/c mice, suggesting an innate response, and levels remained high through to day 14 (time of parasite rejection). Immunohistochemistry of jejunal sections with a rabbit polyclonal Ab to Xenopus laevis 35-kDa cortical granule lectin (XL35; 68% identity with intelectin-2) followed a similar pattern, with intense labeling of goblet and Paneth cells at day 14. However, intelectin-2 transcripts and protein were absent, and immunohistochemistry negative when C57BL/10 mice were infected with T. spiralis. Genomic PCR and Southern blotting confirmed that the intelectin-2 gene is absent from the C57BL/10 genome. The presence of intelectin-2 in resistant BALB/c mice, its absence from the susceptible C57BL/10 strain and the kinetics of its up-regulation during T. spiralis infection suggest that this novel lectin may serve a protective role in the innate immune response to parasite infection.

  • Received January 22, 2004.
  • Accepted May 13, 2004.
  • Copyright © 2004 by The American Association of Immunologists
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The Journal of Immunology: 173 (3)
The Journal of Immunology
Vol. 173, Issue 3
1 Aug 2004
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Innate BALB/c Enteric Epithelial Responses to Trichinella spiralis: Inducible Expression of a Novel Goblet Cell Lectin, Intelectin-2, and Its Natural Deletion in C57BL/10 Mice
Alan D. Pemberton, Pamela A. Knight, John Gamble, William H. Colledge, Jin-Kyu Lee, Michael Pierce, Hugh R. P. Miller
The Journal of Immunology August 1, 2004, 173 (3) 1894-1901; DOI: 10.4049/jimmunol.173.3.1894

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Innate BALB/c Enteric Epithelial Responses to Trichinella spiralis: Inducible Expression of a Novel Goblet Cell Lectin, Intelectin-2, and Its Natural Deletion in C57BL/10 Mice
Alan D. Pemberton, Pamela A. Knight, John Gamble, William H. Colledge, Jin-Kyu Lee, Michael Pierce, Hugh R. P. Miller
The Journal of Immunology August 1, 2004, 173 (3) 1894-1901; DOI: 10.4049/jimmunol.173.3.1894
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