Article Figures & Data
Figures
- FIGURE 1.
Histological and immunopathologic features of renal disease in B6.kd mice. a, Severe interstitial inflammation and cystic dilatation with proliferative glomerulonephritis in a B6.kd mouse at 184 days of age, near end-stage renal disease (H&E-stained paraffin section). b–e, Immunohistological analysis of the cellular infiltrates in the kidneys of a RAG-1−/− kd/kd mouse at 150 days of age, showing lack of staining with an isotype control mAb (b), absence of TCR+ T cells (c), but marked infiltration by CD11b+ macrophages (d) and DX5+ NK cells (e) (cryostat sections, hematoxylin counterstain). f–I, Evaluation of the onset of tubular injury and apoptosis using a mAb to activated caspase-3, showing lacking of staining of a kidney from a 73-day-old control B6 mouse using control mAb (f) or anti-activated caspase-3 mAb (g), whereas a kidney from a 78-day-old B6.kd mouse shows lack of staining with a control mAb (h), but focal tubular labeling (i; arrowheads) for activated caspase-3. Immunohistological data are in each case representative of five kidneys examined from each group at the specified time point; all panels are ×250 original magnification.
Tables
Genotype No. Positive/Total Dissected Days 75–99 Days 100–120 Day >120 B6 NDa 0/3 0/11 B6.kdb 1/11 13/19 34/37 CD4−/−kd/kd 1/4 1/4 22/26 CD8−/−kd/kd ND 8/10 13/17 CD28−/−kd/kd ND 3/19 41/62 IL-2−/−kd/kd 4/9 2/2 ND Rag-1−/−kd/kd 4/8 7/7 21/27 ICAM-1−/−kd/kd 0/2 1/2 23/23 β2m−/−kd/kd 0/2 2/2 8/15 a ND, Not done.
b The B6.kd mice in this table were from at least the eighth generation of backcrossing.
B6.kd CD4−/− kd/kd CD8−/−kd/kd Rag−/−kd/kd CD45+ Diffuse infiltrate with peritubular aggregates 61.6 ± 8.8 cells/HPF Diffuse infiltrate with peritubular aggregates 58.2 ± 7.3 cells/HPF Diffuse infiltrate with peritubular aggregates 64.5 ± 9.2 cells/HPF Diffuse infiltrate with peritubular aggregates 31.2 ± 4.6 cells/HPFb T cells 10–20% of infiltrate 10–20% of infiltrate 10–20% of infiltrate Negative CD4 10–20% of infiltrate Negative 10–20% of infiltrate Negative CD8 1–5% positive 10–20% of infiltrate Negative Negative IL-2R 1–5% positive <1% positive 1–5% positive Negative Mφ >75% leukocytes >75% leukocytes >75% leukocytes ∼50% leukocytes NK ∼10% leukocytes ∼10% leukocytes ∼10% leukocytes ∼50% leukocytes ICOS 5–10% of infiltrate ∼10–20% of infiltrate 1–5% of infiltrate Negative a Assessed in three to four kidneys/group; HPF, high power field; CD45+ cells determined in six consecutive fields/sample (mean ± SD);
b , p < 0.01 vs infiltrates in each of the other three groups listed (t test); additional semiquantitative assessment <1%, 1–5%, 5–10%, 10–20%, 20–50%, 50–75%, or >75%.
In this issue
