Article Figures & Data
Figures
- FIGURE 1.
IFN-γ production by CD8 (A) and CD4 T cells (B) from MyD88-deficient (myd88−/−) and control (myd88+/−) mice following stimulation with the indicated LM-specific peptides or no peptide control. Cells were obtained from indicated mice 7 days after infection with ActA-deficient LM. The numbers in the upper right quadrant indicate the mean percentages (±SE) of IFN-γ-producing CD8 or CD4 T cells. C, Total numbers of IFN-γ-producing CD8 and CD4 T cells per mouse spleen following stimulation with MHC class I- or class II-restricted LM-specific peptides. These data represent seven mice per group from two combined experiments. Bar, SE; ∗, statistically significant difference.
- FIGURE 2.
A, Splenocytes from either MyD88-deficient (myd88−/−) or control (myd88+/+) mice immunized with ActA-deficient LM transfer protection. B, CD8 T cells mediate the protective effects of splenocytes from immunized MyD88-deficient mice. Saline or 6.0 × 107 of the indicated splenocytes were injected into naive C57BL/6 mice 1 h before i.v. infection with 1.0 × 105 CFUs of wt LM 10403s. CFUs of LM in either the spleen (○) or the liver (▵) of mice 3 days after infection were determined. The plot represents the data from five to seven mice per group combined from two separate experiments. Bar, geometric mean.
- FIGURE 3.
Immunization with ActA-deficient LM protects MyD88-deficient (myd88−/−) and control (myd88+/−) mice from infection with wt LM. CFUs of LM in either the spleen (○) or the liver (▵) of MyD88-deficient or control mice 3 days after i.v. challenge with 5.0 × 103 CFUs of wt LM 10403s. The plot represents the data from six to seven mice per group combined from two separate experiments. Bar, geometric mean.
Tables
myd88−/− myd88+/− p Value IFN-γ, unstimulated cells 30.6 ± 30.6 91.2 ± 62.7 0.46 IFN-γ, stimulated cells 3085.6 ± 806.0 11589.4 ± 1427.6 <0.001 IL-4, unstimulated cells n.d. n.d. n.a. IL-4, stimulated cells n.d. n.d. n.a. a Concentrations of IFN-γ and IL-4 in culture supernatants of splenocytes from MyD88-deficient (myd88−/−) and control (myd88+/−) mice following stimulation with the LM class II peptide LLO 189–201. Splenocytes were isolated from these mice 7 days after ActA-deficient LM infection. Data represent the mean (picograms per milliliter) ± SE from seven mice per group from two independent experiments. n.d., not detectable (≤ 37 pg/ml); n.a., not applicable.
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