Article Figures & Data
Figures
- FIGURE 1.
Schematic representation of factor H indicating the locations of the polyanion and C3b-binding sites. Factor H is composed of 20 homologous domains each possessing a similar core structure (45 ). Approximate locations of polyanion binding sites 1, 2, and 3 (24 25 26 27 28 29 ) are indicated at the top, and of the three C3b-binding sites are indicated at the bottom (21 22 23 24 ).
- FIGURE 2.
Lysis of C-activating cells (Er) and host-like polyanion-bearing cells (Es) in factor H-depleted human serum replenished with normal human factor H, with recombinant factor H, or with a deletion mutant of recombinant factor H lacking the C-terminal 10 domains. Human serum depleted of factor H was reconstituted with normal molar concentrations of human factor H (top panel), full-length recombinant factor H made in insect cells (middle panel), or a deletion mutant factor H lacking the C-terminal 10 domains, rHΔ11–20 (bottom panel). Various concentrations of the reconstituted sera were incubated with Er or Es cells for 20 min at 37°C. The assays contained 2.5 mM MgEGTA to inhibit classical and lectin pathway activation and restrict activation to the calcium-independent alternative pathway of C. Lysis was subsequently measured by hemoglobin release after centrifugation to remove unlysed cells.
- FIGURE 3.
Lysis of C-activating cells (Er) and host-like polyanion-bearing cells (Es) in factor H-depleted human serum replenished with one of three recombinant mutants of factor H each lacking a 5-domain region of the 20-domain protein. The assays were performed as described in Fig. 2.
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