Skip to main content

Main menu

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons

User menu

  • Subscribe
  • Log in

Search

  • Advanced search
The Journal of Immunology
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons
  • Subscribe
  • Log in
The Journal of Immunology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Follow The Journal of Immunology on Twitter
  • Follow The Journal of Immunology on RSS

Toll-Like Receptor 2 Is Required for Innate, But Not Acquired, Host Defense to Borrelia burgdorferi

R. Mark Wooten, Ying Ma, R. Alyson Yoder, Jeanette P. Brown, John H. Weis, James F. Zachary, Carsten J. Kirschning and Janis J. Weis
J Immunol January 1, 2002, 168 (1) 348-355; DOI: https://doi.org/10.4049/jimmunol.168.1.348
R. Mark Wooten
*Department of Pathology, University of Utah, Salt Lake City, UT 84132;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ying Ma
*Department of Pathology, University of Utah, Salt Lake City, UT 84132;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
R. Alyson Yoder
*Department of Pathology, University of Utah, Salt Lake City, UT 84132;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jeanette P. Brown
*Department of Pathology, University of Utah, Salt Lake City, UT 84132;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
John H. Weis
*Department of Pathology, University of Utah, Salt Lake City, UT 84132;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
James F. Zachary
†Department of Veterinary Pathobiology, University of Illinois, Urbana, IL 61801; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Carsten J. Kirschning
‡Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Munich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Janis J. Weis
*Department of Pathology, University of Utah, Salt Lake City, UT 84132;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Abstract

Borrelia burgdorferi lipoproteins activate inflammatory cells through Toll-like receptor 2 (TLR2), suggesting that TLR2 could play a pivotal role in the host response to B. burgdorferi. TLR2 does play a critical role in host defense, as infected TLR2−/− mice harbored up to 100-fold more spirochetes in tissues than did TLR2+/+ littermates. Spirochetes persisted at extremely elevated levels in TLR2-deficient mice for at least 8 wk following infection. Infected TLR2−/− mice developed normal Borrelia-specific Ab responses, as measured by quantity of Borrelia-specific Ig isotypes, the kinetics of class switching to IgG, and the complexity of the Ags recognized. These findings indicate that the failure to control spirochete levels in tissues is not due to an impaired acquired immune response. While macrophages from TLR2−/− mice were not responsive to lipoproteins, they did respond to nonlipoprotein components of sonicated spirochetes. These TLR2-independent responses could play a role during the inflammatory response to B. burgdorferi, as infected TLR2−/− mice developed greater ankle swelling than wild-type littermates. Thus, while TLR2-dependent signaling pathways play a major role in the innate host defense to B. burgdorferi, both inflammatory responses and the development of the acquired humoral response can occur in the absence of TLR2.

Borrelia burgdorferi is unique in the abundance and number of tripalmitoyl-S-glyceryl-cysteine-modified lipoproteins synthesized, as >10% of the genome encodes proteins with a predicted signal peptidase II cleavage site (1, 2). These lipoproteins are potent stimulants of proinflammatory products in numerous cell types, including macrophages, endothelial cells, neutrophils, and B lymphocytes (3, 4, 5, 6). Injection of lipoproteins or lipopeptides into joints or skin induces an acute inflammatory infiltrate consisting predominantly of neutrophils (7, 8), suggesting that lipoproteins play a major role in inflammatory induction in vivo. The recent identification of TLR2 as the signal-transducing receptor for bacterial lipoproteins leading to nuclear translocation of the inflammatory transcription factor NF-κB provides a mechanism for the initiation and modification of inflammatory events associated with Lyme disease (3, 9, 10, 11, 12, 13).

Toll-like receptors (TLR)4 are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens; Drosophila toll is required for the anti-fungal response (14), while the related 18-wheeler is involved in antibacterial defenses (15). In mammals, TLR have been implicated in both inflammatory responses and innate host defense to pathogens (16). The best example is the C3H/HeJ mouse, which possess a nonactivating mutation in the gene encoding TLR4, the inflammatory signaling receptor for enterobacterial LPS (17, 18). C3H/HeJ mice are highly resistant to LPS-induced shock while being exquisitely susceptible to the lethal consequences of infection by the LPS-bearing pathogen Salmonella typhimurium (19). Interestingly, B. burgdorferi does not produce LPS (1, 20), and TLR4 appears to play no physiological role in the host defense to B. burgdorferi: C3H/HeJ mice display identical parameters of infection and disease as congenic C3H/HeN mice that possess a wild-type TLR4, including the kinetics of bacterial dissemination, the persistence of spirochetes in tissues, and the pathological severity of arthritis and carditis (21, 22). Furthermore, macrophages from LPS-hyporesponsive C3H/HeJ mice respond strongly to B. burgdorferi lipoproteins and sonicated bacteria (5). These findings suggest that TLR2 could play a unique role in the inflammatory response and host defense to infection with B. burgdorferi, analogous to that of TLR4 during infection with enterobacterial species. This hypothesis was tested by infecting mice possessing a targeted disruption in the tlr2 gene with B. burgdorferi.

Materials and Methods

Mice

C3H/HeN mice were obtained from the National Cancer Institute, and C57BL/6J mice were purchased from The Jackson Laboratory (Bar Harbor, ME). TLR2-deficient mice were generated by replacing a portion of the tlr2 gene with the neomycin resistance gene, oriented in the opposite reading frame (23). These mice do not produce TLR2 protein, and TLR2 transcripts are larger than wild type due to the presence of the neomycin resistance gene (23). Cells from these mice are functionally deficient in TLR2-mediated signaling (see Fig. 1⇓) (23). The TLR2−/− mutation was on a mixed 129SV × C57BL/6 background (see Figs. 1⇓ and 2⇓A) or was backcrossed four generations onto C57BL/6J mice, and heterozygous parents were used to generate experimental TLR2−/−, TLR2+/−, and TLR2+/+ littermates (see Figs. 2⇓B and 3–5 and Tables I–III⇓⇓⇓). Mice were housed in the Animal Resource Center at the University of Utah Medical Center, according to guidelines of the National Institutes of Health for the care and use of laboratory animals.

FIGURE 1.
  • Download figure
  • Open in new tab
  • Download powerpoint
FIGURE 1.

A–D, Macrophages from TLR2-deficient mice fail to respond to B. burgdorferi lipoproteins. Bone marrow-derived macrophages from different mouse strains were stimulated with either recombinant OspA (500 ng/ml) or repurified LPS (100 ng/ml), and 24-h supernatants were assessed for IL-6 (A), IL-10 (C), and TNF-α (D) by ELISA or for nitrate production (B) by Greiss reaction. All samples except those with LPS contained 10 μg/ml polymyxin B.

FIGURE 2.
  • Download figure
  • Open in new tab
  • Download powerpoint
FIGURE 2.

Effect of TLR2 deficiency on macrophages responses to sonicated B. burgdorferi. A, Bone marrow macrophages from TLR2−/− mice and C3H/HeN mice were incubated with the indicated concentrations of OspA or sonicated N40 B. burgdorferi. IL-6 was assayed by ELISA in supernatants collected at 24 h. B, Bone marrow macrophages were prepared from TLR2−/− mice and wild-type littermates and stimulated with the indicated concentrations of OspA or sonicated N40 B. burgdorferi. Supernatants collected at 24 h were assessed for nitrate production by the Greiss reaction. All samples received 10 μg/ml polymyxin B.

View this table:
  • View inline
  • View popup
Table I.

Effect of TLR2 deficiency on ankle pathology in B. burgdorferi-infected mice

View this table:
  • View inline
  • View popup
Table II.

Effect of TRL2-deficiency on the humoral immune response in B. burgdorferi-infected mice

View this table:
  • View inline
  • View popup
Table III.

Distribution of Borrelia-specific Ig isotypes in TLR2-deficient and wild-type mice

Mouse macrophages

Bone marrow-derived macrophages from C3H/HeN, C57BL/6, TLR2−/−, and TLR2+/+ mice were prepared as previously described (24). Briefly, bone marrow cells were cultured in RPMI supplemented with L929-conditioned medium for 7 days at 37°C. Macrophages were recovered with ice-cold PBS and replated in 12-well culture dishes at a density of 5 × 105/well in serum-free medium containing 1% Nutridoma (Roche, Indianapolis, IN). After overnight incubation at 37°C, nonadherent cells were removed, and the indicated agonists were added. Supernatants were harvested from these cultures at the designated times and either assayed immediately (IL-10 and TNF) or stored at −20°C until assayed.

Infection of mice with B. burgdorferi

TLR2−/−, TLR2+/+, and TLR2+/− littermate controls were infected by intradermal injection with 2 × 103 organisms of a passage five culture of the N40 isolate of B. burgdorferi provided by S. Barthold (University of California, Davis, CA) (25).

Measurement of ankle joints

Ankle joint measurements were made as described previously (26). Rear ankle joints were measured at the time of infection and at 2, 4, or 8 wk following infection using a metric caliper, and data were reported as an increase in swelling. Measurements were performed on TLR2−/− mice and littermate controls in a blinded fashion on the thickest portion (anterior-posterior) of the ankle, with the joint extended.

Lesion assessment of ankle joints

Histological analyses were performed on the rear ankle joint that exhibited the greatest swelling from mice sacrificed at 2, 4, or 8 wk postinfection. Joints were fixed in 10% neutral-buffered formalin, decalcified, embedded in paraffin, and sectioned at 5 μm before staining with H&E. Sections were viewed in a blinded fashion and assessed for various lesions of disease, including edema, neutrophil and/or mononuclear inflammation, tendon sheath thickness, and reactive/reparative responses. Each lesion was issued a score ranging from 0 to 5, with 5 representing the most severe lesion and 0 indicating normal tissue. Scores for individual lesions were incorporated into the overall lesion score reported in this study.

Reagents

Sonicated B. burgdorferi was prepared from 10-day cultures as previously described (27). The protein content of this preparation was determined by Bradford assay. Recombinant outer surface protein A (OspA) was provided by J. Dunn (Brookhaven National Laboratories). The recombinant OspA and sonicated B. burgdorferi contained <0.3 endotoxin units/500 ng as determined by Limulus amebocyte assay (Associates of Cape Cod, Cape Cod, MA). Ab and standards for cytokine and Ig ELISAs were obtained from PharMingen (San Diego, CA), and polymyxin B was obtained from Sigma (St. Louis, MO). LPS from Escherichia coli D31 m4 (Re) was purchased from List Biological Laboratories (Campbell, CA) and was repurified according to our previous report (28).

Isolation of DNA from infected mouse tissues

One rear ankle joint, the heart, and ear tissues were harvested from experimental animals sacrificed at 2, 4, or 8 wk postinfection, and DNA was prepared as previously described (22). Briefly, individual tissues were incubated in 0.1% collagenase at 37°C overnight before adding an equal volume of 0.2 mg/ml proteinase K (Sigma). After overnight incubation at 55°C, DNA was recovered by phenol/chloroform extraction and ethanol precipitation. Following digestion with 1 mg/ml DNase-free RNase (Sigma), samples were again extracted, and DNA was recovered by precipitation. This precipitate was resuspended in 1.5 ml water, and DNA content was determined by absorbance at 260 nm.

Quantification of B. burgdorferi by continuous monitoring of PCR

PCR analyses were performed in a fluorescence temperature cycler (LightCycler LC24, Idaho Technology, Idaho Falls, ID) as previously described (29). Briefly, amplification was performed on 200 ng sample DNA in a 10-μl final volume containing 50 mM Tris (pH 8.3), 3 mM MgCl2, 4.5 μg BSA, 200 μM dNTP, a 1/30,000 dilution of SYBR Green I (Molecular Probes, Eugene, OR), 5 μM of each primer, 0.5 U Taq polymerase (Life Technologies, Gaithersburg, MD), and 110 ng TaqStart Ab (Clontech, Palo Alto, CA). Amplification was performed at 40 cycles, with each cycle comprised of heating at 20°C/s to 95°C with a 1-s hold, cooling at 20°C/s to 60°C with a 1-s hold, and heating at 1°C/s to 84°C. This technique continuously monitors the cycle-by-cycle accumulation of fluorescently labeled product. The cycle at which the product is first detected is used as an indicator of the relative starting copy numbers present in the sample. Copy numbers for mouse nidogen and B. burgdorferi recA were calculated using the LightCycler software, and recA values were corrected by normalization based on nidogen copies. The oligonucleotide primers used to detect mouse nidogen were nido.F (5′-CCA GCC ACA GAA TAC CAT CC-3′) and nido.R (5′-GGA CAT ACT CTG CTG CCA TC-3′). The oligonucleotide primers used to detect B. burgdorferi recA were nTM17.F (5′-GTG GAT CTA TTG TAT TAG ATG AGG CTC TCG-3′) and nTM17.R (5′-GCC AAA GTT CTG CAA CAT TAA CAC CTA AAG-3′).

Ig quantification

Serum obtained by retro-orbital bleeding of experimental animals was assayed by ELISA to determine Ig content. Microtiter plates were coated with either sonicated B. burgdorferi or goat Ab to mouse IgG, IgM, and IgA (Life Technologies). Serum dilutions were added to plates for 90 min at 37°C and bound murine Ig was detected by addition of HRP-conjugated Abs to murine IgG or IgM (Zymed, San Francisco, CA). Ig content was estimated by comparing with standard curves using purified IgG or IgM. B. burgdorferi-specific Ig subclasses were assessed by titrating serum samples on plates coated with sonicated B. burgdorferi using isotype-specific HRP-conjugated secondary Abs (Zymed).

Western blot analysis

Western blots were prepared by separating 120 μg sonicated N40 isolate of B. burgdorferi on 12% SDS-PAGE and transferring to Immobilon (Millipore, Bedford, MA). Blots were incubated with a 1/50 dilution of infected or control mouse sera. OspC and OspA were detected with a 1/500 dilution of rabbit polyclonal anti-OspC or 2 μg/ml monoclonal anti-OspA, provided by T. Schwan (Rocky Mountain Laboratories, Hamilton, MT). Bands were detected with alkaline phosphatase-labeled goat anti-mouse or goat anti-rabbit IgG (Life Technologies).

Results

Strong evidence indicates that bacterial lipoproteins activate murine and human cells through TLR2 (9, 10, 11). The prototypic B. burgdorferi lipoprotein, OspA, is a strong stimulant of cytokine production in murine bone marrow-derived macrophages and human cell lines, with responses induced by as little as 1–10 ng/ml OspA (9, 13). As expected, macrophages from TLR2−/− mice failed to respond to 500 ng/ml OspA (Fig. 1⇑, A–C), while responding strongly to repurified LPS (28, 30). The OspA-stimulated production of the proinflammatory products, IL-6 (Fig. 1⇑A), NO (Fig. 1⇑B), and TNF-α (Fig. 1⇑D), was abolished in TLR2-deficient macrophages, as was the production of the anti-inflammatory cytokine IL-10 (Fig. 1⇑C). Thus, lipoprotein signaling through TLR2 is required for both pro- and anti-inflammatory signaling in response to B. burgdorferi lipoproteins, as was previously found for the facilitating molecule CD14 (13, 31, 32).

Because B. burgdorferi lacks LPS but abundantly produces lipoproteins, it has been hypothesized that the majority of cytokine stimulatory activities of the bacteria result from the lipoproteins (33). Macrophages from TLR2-deficient mice were used to test for the presence of additional, TLR2-independent, stimulatory molecules in a sonicated preparation of B. burgdorferi. These experiments demonstrated that the major stimulatory component of B. burgdorferi acts through TLR2, with little response from TLR2-deficient macrophages to 0.5 or 5 μg/ml sonicate (Fig. 2⇑A). However, 50 μg/ml of sonicated B. burgdorferi possessed strong stimulatory activity for macrophages from TLR2−/− mice, although a 10-fold greater concentration of sonicate was required to elicit IL-6 production equivalent to that of macrophages from wild-type C3H/HeN mice (Fig. 2⇑A). The differences in the response of macrophages from C3H/HeN and TLR2-deficient mice (derived from 129SV × C57BL6/J) to sonicated B. burgdorferi (Fig. 2⇑A) could have been influenced by other variations besides the specific disruption of the tlr2 gene. Therefore, macrophages were prepared from TLR2−/− mice and their wild-type littermates for comparison of responses to OspA and sonicated B. burgdorferi. Macrophages from TLR2−/− mice did respond to sonicated B. burgdorferi, but again required 10- to 100-fold more sonicate for equivalent NO production as macrophages from wild-type littermates (Fig. 2⇑B).

The ability of sonicated B. burgdorferi to stimulate responses in macrophages from TLR2-deficient mice is not likely to be due to a second receptor for lipoproteins, as these macrophages failed to respond to 5 μg/ml OspA, a 10-fold higher concentration than the 500 ng/ml found to be optimal in our laboratory (Fig. 2⇑B). Failure of TLR2-deficient macrophages to respond to 5 μg/ml OspA is shown for NO, the most sensitive indicator of macrophage activation, and similar results were obtained with TNF-α, IL-6, and IL-10. The diminished response by wild-type macrophages to 5 μg/ml OspA relative to the more standard maximum concentration of 500 ng/ml (Fig. 2⇑B) was demonstrated to be due to the effects of detergent in the OspA preparation (not shown). These results suggest that the TLR2-independent stimulatory activity in sonicated B. burgdorferi is not mediated by lipoproteins, but, rather, involves additional, less potent microbial products. Numerous products associated with Gram-negative bacteria have been found to signal through various TLR, including peptidoglycan (34), glycolipids (35), unmethylated CpG containing DNA (36), and flagellin (36). Interestingly, the contribution of these nonlipoprotein bacterial compounds to B. burgdorferi stimulation of murine macrophages was only evident in the absence of TLR2 signaling.

The contribution of TLR2 to the development of Lyme disease was determined by infecting TLR2−/− mice with B. burgdorferi and assessing arthritis severity at 4 wk postinfection, the peak of arthritis development. TLR2−/− mice displayed a striking increase in rear ankle swelling compared with their TLR2+/+ and TLR2+/− littermates (Table I⇑). Mice heterozygous for the TLR2 deficiency were similar to homozygous wild-type littermates. The increase in ankle swelling in TLR2−/− mice was also remarkable, as both rear ankle joints displayed uniformly increased swelling, whereas infection of arthritis-susceptible immunocompetent mice typically results in one rear ankle joint being more severely affected (21). Ankle swelling gives only a partial picture of joint pathology in murine Lyme disease, providing information primarily on the degree of edema within the ankle tissue. At sacrifice, the most severely swollen ankle joint was taken for histological analysis to obtain a more complete picture of joint pathology. The C57BL/6 mouse strain typically develops mild to moderate arthritis when infected with B. burgdorferi, with histopathology peaking at about 4 wk following infection. We expected that TLR2 deficiency could result in less severe arthritis, and therefore, its effect might not be dramatic on this mouse background. Lesions within ankle joints were evaluated for disease parameters, including neutrophil infiltration, mononuclear cell infiltration, sheath thickness, reactive and reparative responses, and overall lesion severity. Joints from TLR2−/− mice received slightly higher scores for mononuclear cell infiltration and tendon sheath thickness than did TLR2+/+ and TLR2+/− littermates, However, the scores for these individual parameters and for overall lesion severity for TLR2−/− mice were not statistically different from those for the other two genotypes (Table I⇑). Therefore, on this relatively arthritis-resistant background, TLR2 deficiency did not significantly influence parameters of lesion severity. These findings suggest that the ankle swelling measurements were primarily monitoring edema. The finding that ankle swelling was more dramatically influenced by deletion of TLR2 than was histopathologically scoring of arthritic lesions is consistent with our previous observation that these two traits are regulated independently by genetic loci mapping to distinct locations (37).

The impact of TLR2 on host defense to B. burgdorferi was determined by quantification of spirochetes in DNA prepared from tissues of infected mice 2 and 4 wk postinfection. Hearts, rear ankle joints, and ears were analyzed, as these tissues consistently harbor the highest concentrations of persistent bacteria. All TLR2−/− animals possessed elevated numbers of spirochetes in tissues at both time points tested, ranging up to 100-fold higher in individual TLR2−/− mice than in TLR2+/+ and TLR2+/− littermates (Fig. 3⇓). The impairment to host defense was greatest in rear ankle joints of mice at 2 wk postinfection, with TLR2−/− mice harboring an average of 40-fold more spirochetes than TLR2+/+ mice (p < 0.000001). B. burgdorferi rapidly colonize the skin of infected mice, exhibited by increased numbers in ear tissues from TLR2+/+ and TLR2+/− control mice at 2 wk compared with 4 wk. In contrast, TLR2−/− mice failed to clear the high concentrations of spirochetes from ear tissue by 4 wk, harboring an average of 100-fold more bacteria than littermate controls. Spirochete levels were also much greater in hearts of mice sacrificed at 2 wk than at 4 wk postinfection regardless of the TLR2 genotype. However, at both time points hearts from TLR2−/− mice displayed ∼10-fold more bacteria than the TLR2+/+ or TLR2+/− littermate controls. To determine whether overwhelming systemic infection was underlying the increased presence of tissue spirochetes, the levels of B. burgdorferi in spleens of infected animals were determined. Spleen and blood generally harbor very low numbers of B. burgdorferi, and blood levels are difficult to quantify by PCR. The concentration of B. burgdorferi in the spleens of wild-type mice was extremely low even at the 2 wk peak of tissue spirochetes, (average, 0.05 B. burgdorferi recA genes/1000 copies of the mouse gene nidogen). Although spirochete levels in TLR2-deficient mice (average, 0.34 B. burgdorferi recA genes/1000 nidogen genes) were greater than those in wild-type mice, they were much lower than those found in the three other tissues assayed at 2 wk. Thus, the previous observation that blood and blood-filtering tissues are not major reservoirs for this pathogen (21) is true even in TLR2-deficient mice harboring extremely high levels of bacteria in other tissues. These quantitative differences in spirochete levels in numerous tissues from TLR2−/− mice and littermate controls unequivocally point to a crucial role for TLR2 in the host defense to B. burgdorferi.

FIGURE 3.
  • Download figure
  • Open in new tab
  • Download powerpoint
FIGURE 3.

The host defense to B. burgdorferi is highly dependent on TLR2. Mice were injected with 2 × 103 B. burgdorferi organisms (N40 isolate) intradermally into the shaven back. Tissues were assessed for B. burgdorferi DNA levels in mice sacrificed at 2 or 4 wk postinfection by continuous monitoring PCR (29 ). Values reflect the number of B. burgdorferi recA gene copies normalized per 1000 copies of a single copy murine gene (nidogen); each circle represents an individual animal tissue, while the number beside the bar indicates the average for the group.

The effect of TLR2 deficiency on the long term persistence of B. burgdorferi was assessed by following a small group of infected mice for 8 wk (Fig. 4⇓). Quantitative PCR revealed little change in spirochete levels in hearts, rear ankles, and ears by 8 wk following infection. Thus, the extremely high levels of B. burgdorferi found in tissues from TLR2-deficient mice at 4 wk following infection persisted at 8 wk following infection. Levels in wild-type mice were also similar at 4 and 8 wk following infection. These findings suggest that clearance of spirochetes primarily occurs between 2 and 4 wk, and that spirochetes in wild-type mice may persist at a relatively constant, low level after 4 wk of infection.

FIGURE 4.
  • Download figure
  • Open in new tab
  • Download powerpoint
FIGURE 4.

Spirochetes levels at 8 wk in TLR2-deficient mice. Mice were infected as described in Fig. 3⇑ and were sacrificed 8 wk postinfection. B. burgdorferi DNA was quantified as explained in Fig. 3⇑.

The observation that spirochete concentrations in tissues drop between 2 and 4 wk of infection in immunocompetent mice (as seen in Fig. 3⇑ for TLR2+/− and TLR2+/+ mice) has been attributed to the appearance of acquired immunologic defenses, particularly Ab directed to surface exposed proteins of B. burgdorferi. Importantly, in mice with the scid mutation, spirochete numbers remain elevated at later time points, and passive transfer of Abs can reduce spirochete concentrations in tissues, supporting the role of acquired defenses in clearing infection and resolving disease (38, 39, 40). We hypothesized that the persistently elevated concentrations of spirochetes in tissues from TLR2−/− mice could result from defective development of an Ab response. Serum was collected from infected mice at 2, 4, and 8 wk following infection, and the amount of B. burgdorferi-specific IgM and IgG was determined by capture ELISA. Surprisingly, TLR2−/− mice produced similar levels of anti-B. burgdorferi IgM at 2 wk and of IgG at 4 and 8 wk as their littermates controls (Table II⇑). Infection of mice with B. burgdorferi has been associated with an increase in total circulating IgG (26). Uninfected mice possess 1–2 mg/ml circulating IgG, while in infected mice the level of total serum IgG increased 5- to 10-fold, but was similar for all genotypes of infected mice at both 4 and 8 wk (Table II⇑).

The absence of a particular subclass of Ab could result in the poor control of spirochetes in tissues. To assess this possibility, isotype distribution of Borrelia-specific Ab was determined in sera collected 4 wk following infection. IgG1, IgG2b, and IgG3 isotypes are produced by C57BL/6 mice, while IgG2a is not (41). As shown in Table III⇑, there was no major shift in the distribution of IgG isotypes in mice of different genotypes, although IgG2b was somewhat under-represented in sera from infected TLR2−/− mice. However, the animal-to-animal variation within groups for Borrelia-specific IgG2b was larger than the average differences between genotypes, resulting in a lack of statistical significance. This strongly argues against under-representation of a particular subclass of anti-Borrelia IgG in TLR2−/− mice that could be responsible for the increased numbers of spirochetes in tissues. The lack of influence on isotype is in sharp contrast to the dramatic effect of IL-4 gene disruption on IgG1 production in B. burgdorferi-infected mice, a finding detected using this assay (42).

The effect of TLR2 deficiency on the spectrum of B. burgdorferi-specific Ags recognized by the humoral immune response was assessed by Western blot analysis, using a preparation of the N40 culture. The complexity of Ags recognized by sera from TLR2−/− mice was similar to that from infected TLR2+/+ mice (Fig. 5⇓). In fact, the number of bands detected and the intensity of staining were actually greater with sera from TLR-deficient mice than with those from wild-type littermates at both 4 and 8 wk postinfection (Fig. 5⇓, A and B). This is likely to reflect the much greater levels of spirochetes, and therefore Ags, present in numerous tissues from TLR2-deficient mice. The presence of Abs against two well-characterized Borrelia Ags, OspA and OspC, was also assessed by Western blot analysis. Control lanes were developed with monoclonal or polyclonal Abs directed against OspA or OspC, respectively, and indicated that these Ags were present in the Ag preparation (Fig. 5⇓). Infected mouse sera from both genotypes of mice contained Ig that reacted with proteins migrating in the same range as OspA and OspC. However, when the control and infected mouse sera were used in Western blots using recombinant OspA and OspC, reactivity was detected with the control anti-OspA or anti-OspC Abs, but not with the infected mouse sera (not shown). Although cultured bacteria do not represent the totality of Ags expressed by spirochetes within the mammal, this sampling of Ags is likely to reveal major alterations in Ags recognized by TLR2-deficient mice. The Western blot results indicate that the complexity of the humoral response is not reduced by deficiency in TLR2, and that gross absence of Ab directed to immunological proteins is not responsible for the observed failure to clear the spirochetes.

FIGURE 5.
  • Download figure
  • Open in new tab
  • Download powerpoint
FIGURE 5.

Western blot analysis of sera from TLR2-deficient and wild-type mice. Abs directed against B. burgdorferi Ags were detected by Western blot analysis as described in Materials and Methods. The positions of OspA and OspC were detected with monoclonal anti-OspA and rabbit polyclonal anti-OspC, respectively. Sera were collected from mice sacrificed at 4 wk (A) or 8 wk (B) postinfection. A, Sera were from two uninfected mice (one each, TLR2+/+ and TLR2+/−), four of the 4 wk infected TLR2+/+ mice, or four of the 4 wk infected TLR2−/− mice, randomly selected from those shown in Fig. 3⇑. B, Sera were from two uninfected mice (one each, TLR2+/+ and TLR2+/−) or from all of the 8 wk infected TLR2+/+ mice and TLR2−/− mice shown in Fig. 4⇑. The positions of molecular mass markers are indicated.

Discussion

B. burgdorferi are atypical Gram-negative bacteria in that they lack LPS and abundantly express Pam3Cys-modified proteins. We and others previously demonstrated that TLR2 is the signaling receptor for bacterial lipoproteins, including the prototypic Borrelia lipoprotein OspA (9, 10, 11). It is well documented that LPS-hyporesponsive mice with a mutant TLR4 are more susceptible to lethal infection with S. typhimurium while being resistant to LPS-induced shock (19) This suggested that TLR2-mediated signaling could play a similarly complex role in both the inflammatory response and the host defense to B. burgdorferi. This hypothesis was tested in mice with a targeted disruption in tlr2. A deficiency in TLR2 greatly impaired the host defense to B. burgdorferi, resulting in up to 100-fold more spirochetes in tissues than those in wild-type littermates. An increased presence of B. burgdorferi was found in three different tissues (ankle joints, ears, and hearts) at three different time points (2, 4, and 8 wk) following infection (Figs. 3⇑ and 4⇑). A fourth tissue, the spleen, was assayed at 2 wk following infection, and TLR2−/− mice were found to harbor ∼7-fold more spirochetes than wild-type littermates. These findings present a comprehensive picture of a host severely compromised in its ability to regulate spirochete levels in tissues.

These results provide hints about the normal mechanism of host control of spirochetes in tissues. In the infected wild-type mice, levels of tissue spirochetes peak at about 2 wk following infection (43), and drop in heart and ears by 4 wk following infection. Spirochete levels in ankles of wild-type mice remained relatively constant at all three time points: 8, 19, and 11 spirochetes/1000 host genomes. These findings are dramatically demonstrated by the data presented in Figs. 3⇑ and 4⇑. In TLR2-deficient mice, the 2 wk levels of tissue spirochetes were even more elevated than in wild-type mice and remained abnormally high at 4 and 8 wk following infection. Several studies have demonstrated a critical role of Borrelia-specific Ig in the protective host defense to B. burgdorferi (38, 39, 40). These results are consistent with those findings, as the anti-B. burgdorferi humoral response at 2 wk is characterized by low level of IgM (26). By 4 wk following infection higher concentrations of Borrelia-specific IgG appear (21) (Table II⇑). This suggests that the drop in spirochete number seen in wild-type mice between 2 and 4 wk following infection is due to the appearance of IgG Abs isotypes.

B. burgdorferi lipoproteins have been reported to possess potent B cell mitogenic properties capable of stimulating polyclonal activation of proliferation and Ig production in vitro (5). These properties as well as the adjuvant properties of lipoprotein vaccines are dependent on the Pam3Cys modification (44, 45). We hypothesized that the humoral response would be altered in TLR2−/− mice, and that this was responsible for the failure to control tissue spirochetes. A complete analysis of the Ab response revealed virtually no difference between TLR2−/− mice and TLR2+/+ littermates; the kinetics of appearance of IgM and class switching to IgG appeared to be normal, the magnitude of IgM and IgG response to B. burgdorferi Ags was similar, the relative distribution of IgG isotypes was the same, and the complexity of Ags recognized was similar. In fact, the findings presented in Tables II⇑ and III⇑ and Fig. 5⇑ fail to support any compromise in the humoral response to B. burgdorferi in TLR2-deficient mice and indicate that the increased number of spirochetes found in tissues of TLR2-deficient mice cannot be attributed to a defective humoral immune response.

The finding that the host defense to B. burgdorferi in TLR2-deficient mice is severely compromised even though anti-spirochete IgG levels are normal argues that effective elimination of B. burgdorferi requires not only Ab, but also a TLR2-expressing effector cell. Phagocytic cells, such as neutrophils and macrophages, are probably TLR2-expressing cells types that could mediate the Ab-dependent clearance of B. burgdorferi (46). Neutrophils have been shown to play a critical role in spirochete clearance (47), and they may be very important in controlling numbers of Ab-opsonized B. burgdorferi in TLR2-deficient mice. The complete lack of in vitro response of cells from TLR2−/− mice to purified lipoproteins (Figs. 1⇑ and 2⇑) (23) suggests that TLR2-mediated activation of inflammatory cells is critical to the control of spirochete numbers in tissues. Interestingly, there was some clearance from the hearts between 2 and 4 wk in TLR2−/− mice that was not observed in ear tissues and ankle joints. Others have suggested that the inflammatory infiltrate in hearts of infected mice is predominated by monocytic cells, whereas the infiltrate in joints is predominately neutrophil (48). Thus, understanding the TLR2-dependent control of host defense could provide insight into the tissue-specific control of B. burgdorferi.

An unexpected finding from this study was the ability of macrophages from TLR2-deficient mice to respond to nonlipoprotein components of B. burgdorferi. The TLR2-lipoprotein interaction clearly dominates the macrophage response to this spirochete; however, the unique situation of the TLR2−/− mouse allows detection of the effects of other stimulatory molecules. Although the significance during infection of TLR2-independent signaling events during infection remains to be determined, the fact that environmental regulation of lipoprotein gene expression is certainly involved in the persistence and pathogenesis of this organism suggest potential importance during selective stages of infection. The finding that the humoral response to B. burgdorferi was normal in TLR2-deficient mice argues that TLR2-independent signaling supports B cell activation in mice. This was surprising due to the complete dependence of the OspA vaccine on the Pam3Cys modification (44). Whether the humoral response requires signals from other TLR or reflects Ag uptake and processing pathways distinct from TLR cannot be determined from these studies. The possible involvement of other TLR is suggested by the wide variety of microbial products that are able to mediate macrophage activation. Several of these signal through TLR2 (12) and therefore should not contribute to the TLR2-independent activation. Some of these may signal through TLR4, such as cellular glycolipids (35), although the in vivo significance of TLR4 signaling is questionable, as C3H/HeJ mice develop disease that is indistinguishable from that in C3H/HeN mice (21, 22). One candidate for nonlipoprotein stimulatory activity in sonicated B. burgdorferi is unmethylated CpG DNA, which has recently been shown to activate murine cells through TLR9 (36). Additionally, flagella from two diverse bacteria, Listeria monoctyogenes and S. typhimurium, have recently been found to signal through TLR5, suggesting that Borrelia flagellin could also contribute to the TLR2-independent response (49). The identification of spirochetal products responsible for TLR2-independent signaling will require further studies, as will determination of the relative physiological importance of such host-pathogen interactions. The complexity of TLR involvement has been recently observed in the macrophage response to Staphylococcus aureus, suggesting that the integration of multiple signaling receptors may be a common theme in bacterial pathogenesis (50).

Although TLR2-independent responses require significantly higher concentrations of bacteria than do TLR2-dependent responses in cell cultures, these stimuli could be important in vivo, where localized concentrations of bacteria can be relatively high. For example, ankle joints from TLR2−/− mice not only harbor an average of 10-fold more spirochetes than joints from wild-type mice, but also display increased ankle swelling relative to littermate controls. This finding clearly demonstrates that a pattern recognition receptor independent of TLR2 can contribute to B. burgdorferi-induced lesions in vivo. A more complete picture of the involvement of TLR2 in inflammatory arthritis development will require studies in which the TLR2 deficiency has been crossed onto a mouse strain that develops more severe Lyme arthritis.

The results presented in this study give a clear picture of TLR2 involvement in control of spirochete levels in tissues, but not in the development of the acquired humoral response. This suggests that the lipoprotein-TLR2 interaction plays a crucial role in activating cells of the innate defense for infiltration, phagocytosis, or killing of spirochetes, and that spirochete ligands for additional TLRs cannot compensate for this interaction. In contrast, the Ab response appeared normal, suggesting that TLR2-independent activation of B lymphocytes functions efficiently in the absence of TLR2. It has been reported with human B lymphocytes that TLR2 is expressed by activated B lymphocytes within germinal centers, but is not expressed by resting B lymphocytes (46, 51). It has also been reported that accessory cells are required for lipoprotein-mediated polyclonal activation of B lymphocytes (52). These results suggests that B cell activation by lipoproteins may be subsequent to activation of accessory cells, which may express numerous TLR. Our results demonstrate that innate defenses to B. burgdorferi are more severely compromised by TLR2 deficiency than are acquired defenses and suggest a new paradigm for host-pathogen interactions.

Acknowledgments

We thank John Dunn at Brookhaven National Laboratories for providing the recombinant OspA and Tom Schwan at the Rocky Mountain Laboratories for the anti-OspA and anti-OspC.

Footnotes

  • ↵1 This work was supported by Public Health Service Grants AI32223 and AI43521 (to J.J.W.), AI24158 (to J.H.W.), and 5P30CA42014 (to University of Utah) and by funds from Associated Regional University Pathologists. The project was also supported in part by an award from the American Lung Association (to J.H.W.) and an Arthritis Foundation Postdoctoral Fellowship (to R.M.W.).

  • ↵2 Current address: Department of Microbiology and Immunology, Medical College of Ohio, Health Education Building, Room 254, 3055 Arlington Avenue, Toledo, OH 43614-5806.

  • ↵3 Address correspondence and reprint requests to Dr. Janis J. Weis, Department of Pathology, University of Utah School of Medicine, 50 North Medical Drive, Salt Lake City, UT 84132. E-mail address: janis.weis{at}path.utah.edu

  • ↵4 Abbreviations used in this paper: TLR, toll-like receptor; OspA, outer surface protein A.

  • Received September 5, 2001.
  • Accepted October 22, 2001.
  • Copyright © 2002 by The American Association of Immunologists

References

  1. ↵
    Fraser, C. M., S. Casjens, W. M. Huang, G. G. Sutton, R. Clayton, R. Lathigra, O. White, K. A. Ketchum, R. Dodson, E. K. Hickey, et al 1997. Genomic sequence of a Lyme disease spirochaete Borrelia burgdorferi. Nature 390: 580
    OpenUrlCrossRefPubMed
  2. ↵
    Casjens, S., N. Palmer, R. van Vugt, W. M. Huang, B. Stevenson, P. Rosa, R. Lathigra, G. Sutton, J. Peterson, R. J. Dodson, et al 2000. A bacterial genome in flux: the twelve linear and nine circular extrachromosomal DNAs in an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi. Mol. Microbiol. 35: 490
    OpenUrlCrossRefPubMed
  3. ↵
    Wooten, R. M., V. R. Modur, T. M. McIntyre, J. J. Weis. 1996. Borrelia burgdorferi outer membrane protein A induces nuclear translocation of nuclear factor-κB and inflammatory activation in human endothelial cells. J. Immunol. 157: 4584
    OpenUrlAbstract
  4. ↵
    Morrison, T. B., J. H. Weis, J. J. Weis. 1997. Borrelia burgdorferi outer surface protein A (OspA) activates and primes human neutrophils. J. Immunol. 158: 4838
    OpenUrlAbstract
  5. ↵
    Ma, Y., J. J. Weis. 1993. Borrelia burgdorferi outer surface lipoproteins OspA and OspB possess B-cell mitogenic and cytokine-stimulatory properties. Infect. Immun. 61: 3843
    OpenUrlAbstract/FREE Full Text
  6. ↵
    Radolf, J. D., L. L. Arndt, D. R. Akins, L. L. Curetty, M. E. Levi, Y. Shen, L. S. Davis, M. V. Norgard. 1995. Treponema pallidum and Borrelia burgdorferi lipoproteins and synthetic lipopeptides activate monocytes/macrophages. J. Immunol. 154: 2866
    OpenUrlAbstract
  7. ↵
    Gondolf, K. B., M. Mihatsch, E. Curschellas, J. J. Dunn, S. R. Batsford. 1994. Induction of experimental allergic arthritis with outer surface proteins of Borrelia burgdorferi. Arthritis Rheum. 37: 1070
    OpenUrlCrossRefPubMed
  8. ↵
    Norgard, M. V., B. S. Riley, J. A. Richardson, J. D. Radolf. 1995. Dermal inflammation elicited by synthetic analogs of Treponema pallidum and Borrelia burgdorferi lipoproteins. Infect. Immun. 63: 1507
    OpenUrlAbstract/FREE Full Text
  9. ↵
    Hirschfeld, M., C. J. Kirschning, R. Schwandner, H. Wesche, J. H. Weis, R. M. Wooten, J. J. Weis. 1999. Cutting edge: inflammatory signaling by Borrelia burgdorferi lipoproteins is mediated by Toll-like receptor 2. J. Immunol. 163: 2382
    OpenUrlAbstract/FREE Full Text
  10. ↵
    Aliprantis, A. O., R. B. Yang, M. R. Mark, S. Suggett, B. Devaux, J. D. Radolf, G. R. Klimpel, P. Godowski, A. Zychlinsky. 1999. Cell activation and apoptosis by bacterial lipoproteins through Toll-like receptor-2. Science 285: 736
    OpenUrlAbstract/FREE Full Text
  11. ↵
    Brightbill, H. D., D. H. Libraty, S. R. Krutzik, R. B. Yang, J. T. Belisle, J. R. Bleharski, M. Maitland, M. V. Norgard, S. E. Plevy, S. T. Smale, et al 1999. Host defense mechanisms triggered by microbial lipoproteins through Toll-like receptors. Science 285: 732
    OpenUrlAbstract/FREE Full Text
  12. ↵
    Lien, E., T. J. Sellati, A. Yoshimura, T. H. Flo, G. Rawadi, R. W. Finberg, J. D. Carroll, T. Espevik, R. R. Ingalls, J. D. Radolf, et al 1999. Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products. J. Biol. Chem. 274: 33419
    OpenUrlAbstract/FREE Full Text
  13. ↵
    Wooten, R. M., T. B. Morrison, J. H. Weis, S. D. Wright, R. Thieringer, J. J. Weis. 1998. The role of CD14 in signaling mediated by outer membrane lipoproteins of Borrelia burgdorferi. J. Immunol. 160: 5485
    OpenUrlAbstract/FREE Full Text
  14. ↵
    Lemaitre, B., E. Nicolas, L. Michaut, J. M. Reichhart, J. A. Hoffmann. 1996. The dorsoventral regulatory gene cassette spatzle/Toll/cactus controls the potent antifungal response in Drosophila adults. Cell 86: 973
    OpenUrlCrossRefPubMed
  15. ↵
    Lemaitre, B., J. M. Reichhart, J. A. Hoffmann. 1997. Drosophila host defense: differential induction of antimicrobial peptide genes after infection by various classes of microorganisms. Proc. Natl. Acad. Sci. USA 94: 14614
    OpenUrlAbstract/FREE Full Text
  16. ↵
    O’Neill, L. A., C. A. Dinarello. 2000. The IL-1 receptor/Toll-like receptor superfamily: crucial receptors for inflammation and host defense. Immunol. Today 21: 206
    OpenUrlCrossRefPubMed
  17. ↵
    Qureshi, S. T., L. Larivire, G. Leveque, S. Clermont, K. J. Moore, P. Gros, D. Malo. 1999. Endotoxin-tolerant mice have mutations in Toll-like receptor 4 (Tlr4). J. Exp. Med. 189: 615
    OpenUrlAbstract/FREE Full Text
  18. ↵
    Poltorak, A., X. He, I. Smirnova, M. Y. Liu, C. V. Huffel, X. Du, D. Birdwell, E. Alejos, M. Silva, C. Galanos, et al 1998. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science 282: 2085
    OpenUrlAbstract/FREE Full Text
  19. ↵
    O’Brien, A. D., D. L. Rosenstreich, I. Scher, G. H. Campbell, R. P. MacDermott, S. B. Formal. 1980. Genetic control of susceptibility to Salmonella typhimurium in mice: role of the LPS gene. J. Immunol. 124: 20
    OpenUrlPubMed
  20. ↵
    Takayama, K., R. J. Rothenberg, A. G. Barbour. 1987. Absence of lipopolysaccharide in the Lyme disease spirochete Borrelia burgdorferi. Infect. Immun. 55: 2311
    OpenUrlAbstract/FREE Full Text
  21. ↵
    Barthold, S. W., D. H. Persing, A. L. Armstrong, R. A. Peeples. 1991. Kinetics of Borrelia burgdorferi dissemination and evolution of disease after intradermal inoculation of mice. Am. J. Pathol. 139: 263
    OpenUrlPubMed
  22. ↵
    Ma, Y., K. P. Seiler, E. J. Eichwald, J. H. Weis, C. Teuscher, J. J. Weis. 1998. Distinct characteristics of resistance to Borrelia burgdorferi-induced arthritis in C57BL/6N mice. Infect. Immun. 66: 161
    OpenUrlAbstract/FREE Full Text
  23. ↵
    Werts, C., R. I. Tapping, J. C. Mathison, T. H. Chuang, V. Kravchenko, I. Saint Girons, D. A. Haake, P. J. Godowski, F. Hayashi, A. Ozinsky, et al 2001. Leptospiral lipopolysaccharide activates cells through a TLR2-dependent mechanism. Nat. Immunol. 2: 346
    OpenUrlCrossRefPubMed
  24. ↵
    Brown, J. P., J. F. Zachary, C. Teuscher, J. J. Weis, R. M. Wooten. 1999. Dual role of interleukin-10 in murine Lyme disease: regulation of arthritis severity and host defense. Infect. Immun. 67: 5142
    OpenUrlAbstract/FREE Full Text
  25. ↵
    Barthold, S. W., D. S. Beck, G. M. Hansen, G. A. Terwilliger, K. D. Moody. 1990. Lyme borreliosis in selected strains and ages of laboratory mice. J. Infect. Dis. 162: 133
    OpenUrlAbstract/FREE Full Text
  26. ↵
    Yang, L., Y. Ma, R. Schoenfeld, M. Griffiths, E. Eichwald, B. Araneo, J. J. Weis. 1992. Evidence for B-lymphocyte mitogen activity in Borrelia burgdorferi-infected mice. Infect. Immun. 60: 3033
    OpenUrlAbstract/FREE Full Text
  27. ↵
    Schoenfeld, R., B. Araneo, Y. Ma, L. M. Yang, J. J. Weis. 1992. Demonstration of a B-lymphocyte mitogen produced by the Lyme disease pathogen. Borrelia burgdorferi. Infect. Immun. 60: 455
    OpenUrlAbstract/FREE Full Text
  28. ↵
    Hirschfeld, M., Y. Ma, J. H. Weis, S. N. Vogel, J. J. Weis. 2000. Repurification of LPS eliminates signaling through both human and murine Toll-like receptor 2. J. Immunol. 165: 618
    OpenUrlAbstract/FREE Full Text
  29. ↵
    Morrison, T. B., Y. Ma, J. H. Weis, J. J. Weis. 1999. Rapid and sensitive quantification of Borrelia burgdorferi-infected mouse tissues by continuous fluorescent monitoring of PCR. J. Clin. Microbiol. 37: 987
    OpenUrlAbstract/FREE Full Text
  30. ↵
    Tapping, R. I., S. Akashi, K. Miyake, P. J. Godowski, P. S. Tobias. 2000. Toll-like receptor 4, but not Toll-like receptor 2, is a signaling receptor for Escherichia and Salmonella lipopolysaccharides. J. Immunol. 165: 5780
    OpenUrlAbstract/FREE Full Text
  31. ↵
    Giambartolomei, G. H., V. A. Dennis, B. L. Lasater, M. T. Philipp. 1999. Induction of pro- and anti-inflammatory cytokines by Borrelia burgdorferi lipoproteins in monocytes is mediated by CD14. Infect. Immun. 67: 140
    OpenUrlAbstract/FREE Full Text
  32. ↵
    Sellati, T. J., D. A. Bouis, R. L. Kitchens, R. P. Darveau, J. Pugin, R. J. Ulevitch, S. C. Gangloff, S. M. Goyert, M. V. Norgard, J. D. Radolf. 1998. Treponema pallidum and Borrelia burgdorferi lipoproteins and synthetic lipopeptides activate monocytic cells via a CD14-dependent pathway distinct from that used by lipopolysaccharide. J. Immunol. 160: 5455
    OpenUrlAbstract/FREE Full Text
  33. ↵
    Wooten, R. M., J. J. Weis. 2001. Host-pathogen interactions promoting inflammatory Lyme arthritis: use of mouse models for dissection of disease processes. Curr. Opin. Microbiol. 4: 274
    OpenUrlCrossRefPubMed
  34. ↵
    Schwandner, R., R. Dziarski, H. Wesche, M. Rothe, C. J. Kirschning. 1999. Peptidoglycan- and lipoteichoic acid-induced cell activation is mediated by Toll-like receptor 2. J. Biol. Chem. 274: 17406
    OpenUrlAbstract/FREE Full Text
  35. ↵
    Hashimoto, M., Y. Imamura, T. Morichika, K. Arimoto, O. Takeuchi, K. Takeda, S. Akira, K. Aoyama, T. Tamura, S. Kotani, et al 2000. Cytokine-inducing macromolecular glycolipids from Enterococcus hirae: improved method for separation and analysis of its effects on cellular activation. Biochim. Biophys. Acta 273: 164
    OpenUrl
  36. ↵
    Hemmi, H., O. Takeuchi, T. Kawai, T. Kaisho, S. Sato, H. Sanjo, M. Matsumoto, K. Hoshino, H. Wagner, K. Takeda, et al 2000. A Toll-like receptor recognizes bacterial DNA. Nature 408: 740
    OpenUrlCrossRefPubMed
  37. ↵
    Weis, J. J., B. A. McCracken, Y. Ma, D. Fairbairn, R. J. Roper, T. B. Morrison, J. H. Weis, J. F. Zachary, R. W. Doerge, C. Teuscher. 1999. Identification of quantitative trait loci governing arthritis severity and humoral responses in the murine model of Lyme disease. J. Immunol. 162: 948
    OpenUrlAbstract/FREE Full Text
  38. ↵
    Barthold, S. W., C. L. Sidman, A. L. Smith. 1992. Lyme borreliosis in genetically resistant and susceptible mice with severe combined immunodeficiency. Am. J. Trop. Med. Hyg. 47: 605
    OpenUrlAbstract/FREE Full Text
  39. ↵
    Schaible, U. E., R. Wallich, M. D. Kramer, G. Nerz, T. Stehle, C. Museteanu, M. M. Simon. 1994. Protection against Borrelia burgdorferi infection in SCID mice is conferred by presensitized spleen cells and partially by B but not T cells alone. Int. Immunol. 6: 671
    OpenUrlAbstract/FREE Full Text
  40. ↵
    Barthold, S. W., M. deSouza, S. Feng. 1996. Serum-mediated resolution of Lyme arthritis in mice. Lab. Invest. 74: 57
    OpenUrlPubMed
  41. ↵
    Martin, R. M., A. M. Lew. 1998. Is IgG2a a good Th1 marker in mice?. Immunol. Today 19: 49
    OpenUrlPubMed
  42. ↵
    Potter, M. R., N. Noben-Trauth, J. H. Weis, C. Teuscher, J. J. Weis. 2000. Interleukin-4 (IL-4) and IL-13 signaling pathways do not regulate Borrelia burgdorferi-induced arthritis in mice: IgG1 is not required for host control of tissue spirochetes. Infect. Immun. 68: 5603
    OpenUrlAbstract/FREE Full Text
  43. ↵
    Yang, L., J. H. Weis, E. Eichwald, C. P. Kolbert, D. H. Persing, J. J. Weis. 1994. Heritable susceptibility to severe Borrelia burgdorferi-induced arthritis is dominant and is associated with persistence of large numbers of spirochetes in tissues. Infect. Immun. 62: 492
    OpenUrlAbstract/FREE Full Text
  44. ↵
    Erdile, L. F., M. A. Brandt, D. J. Warakomski, G. J. Westrack, A. Sädziene, A. G. Barbour, J. P. Mays. 1993. Role of attached lipid in immunogenicity of Borrelia burgdorferi OspA. Infect. Immun. 61: 81
    OpenUrlAbstract/FREE Full Text
  45. ↵
    Weis, J. J., Y. Ma, L. F. Erdile. 1994. Biological activities of native and recombinant Borrelia burgdorferi outer surface protein A: dependence on lipid modification. Infect. Immun. 62: 4632
    OpenUrlAbstract/FREE Full Text
  46. ↵
    Muzio, M., N. Polentarutti, D. Bosisio, P. P. Manoj Kumar, A. Mantovani. 2000. Toll-like receptor family and signalling pathway. Biochem. Soc. Trans. 28: 563
    OpenUrlAbstract/FREE Full Text
  47. ↵
    Barthold, S. W., M. de Souza. 1995. Exacerbation of Lyme arthritis in beige mice. J. Infect. Dis. 172: 778
    OpenUrlAbstract/FREE Full Text
  48. ↵
    Ruderman, E. M., J. S. Kerr, S. R. Telford, A. I. I. I., L. H. Spielman, L. H. Glimcher, E. M. Gravallese. 1995. Early murine Lyme carditis has a macrophage predominance and is independent of major histocompatibility complex class II-CD4+ T cell interactions. J. Infect. Dis. 171: 362
    OpenUrlAbstract/FREE Full Text
  49. ↵
    Hayashi, F., K. D. Smith, A. Ozinsky, T. R. Hawn, E. C. Yi, D. R. Goodlett, J. K. Eng, S. Akira, D. M. Underhill, A. Aderem. 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature 410: 1099
    OpenUrlCrossRefPubMed
  50. ↵
    Takeuchi, O., K. Hoshino, S. Akira. 2000. Cutting edge: TLR2-deficient and MyD88-deficient mice are highly susceptible to Staphylococcus aureus infection. J. Immunol. 165: 5392
    OpenUrlAbstract/FREE Full Text
  51. ↵
    Flo, T. H., O. Halaas, S. Torp, L. Ryan, E. Lien, B. Dybdahl, A. Sundan, T. Espevik. 2001. Differential expression of Toll-like receptor 2 in human cells. J. Leukocyte Biol. 69: 474
    OpenUrlAbstract/FREE Full Text
  52. ↵
    Snapper, C. M., F. R. Rosas, L. Jin, C. Wortham, M. R. Kehry, J. J. Mond. 1995. Bacterial lipoproteins may substitute for cytokines in the humoral immune response to T cell-independent type II antigens. J. Immunol. 155: 5582
    OpenUrlAbstract/FREE Full Text
View Abstract
PreviousNext
Back to top

In this issue

The Journal of Immunology: 168 (1)
The Journal of Immunology
Vol. 168, Issue 1
1 Jan 2002
  • Table of Contents
  • About the Cover
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about The Journal of Immunology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Toll-Like Receptor 2 Is Required for Innate, But Not Acquired, Host Defense to Borrelia burgdorferi
(Your Name) has forwarded a page to you from The Journal of Immunology
(Your Name) thought you would like to see this page from the The Journal of Immunology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Toll-Like Receptor 2 Is Required for Innate, But Not Acquired, Host Defense to Borrelia burgdorferi
R. Mark Wooten, Ying Ma, R. Alyson Yoder, Jeanette P. Brown, John H. Weis, James F. Zachary, Carsten J. Kirschning, Janis J. Weis
The Journal of Immunology January 1, 2002, 168 (1) 348-355; DOI: 10.4049/jimmunol.168.1.348

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Toll-Like Receptor 2 Is Required for Innate, But Not Acquired, Host Defense to Borrelia burgdorferi
R. Mark Wooten, Ying Ma, R. Alyson Yoder, Jeanette P. Brown, John H. Weis, James F. Zachary, Carsten J. Kirschning, Janis J. Weis
The Journal of Immunology January 1, 2002, 168 (1) 348-355; DOI: 10.4049/jimmunol.168.1.348
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Early Self-Regulatory Mechanisms Control the Magnitude of CD8+ T Cell Responses Against Liver Stages of Murine Malaria
  • Sublethal Hyperoxia Impairs Pulmonary Innate Immunity
  • Dependence of IL-4, IL-13, and Nematode-Induced Alterations in Murine Small Intestinal Smooth Muscle Contractility on Stat6 and Enteric Nerves
Show more HOST DEFENSE

Similar Articles

Navigate

  • Home
  • Current Issue
  • Next in The JI
  • Archive
  • Brief Reviews
  • Pillars of Immunology
  • Translating Immunology

For Authors

  • Submit a Manuscript
  • Instructions for Authors
  • About the Journal
  • Journal Policies
  • Editors

General Information

  • Advertisers
  • Subscribers
  • Rights and Permissions
  • Accessibility Statement
  • Public Access
  • Privacy Policy
  • Disclaimer

Journal Services

  • Email Alerts
  • RSS Feeds
  • ImmunoCasts
  • Twitter

Copyright © 2021 by The American Association of Immunologists, Inc.

Print ISSN 0022-1767        Online ISSN 1550-6606