Skip to main content

Main menu

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
      • Neuroimmunology: To Sense and Protect
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons

User menu

  • Subscribe
  • Log in

Search

  • Advanced search
The Journal of Immunology
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons
  • Subscribe
  • Log in
The Journal of Immunology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Follow The Journal of Immunology on Twitter
  • Follow The Journal of Immunology on RSS

Adenoviral Delivery of Human and Viral IL-10 in Murine Sepsis

Rebecca M. Minter, Maria A. Ferry, Michelle E. Murday, Cynthia L. Tannahill, Frances R. Bahjat, Caroline Oberholzer, Andreas Oberholzer, Drake LaFace, Beth Hutchins, Shufen Wen, Jeremy Shinoda, Edward M. Copeland III and Lyle L. Moldawer
J Immunol July 15, 2001, 167 (2) 1053-1059; DOI: https://doi.org/10.4049/jimmunol.167.2.1053
Rebecca M. Minter
*Department of Surgery, University of Florida College of Medicine, Gainesville, FL 32610; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maria A. Ferry
*Department of Surgery, University of Florida College of Medicine, Gainesville, FL 32610; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michelle E. Murday
*Department of Surgery, University of Florida College of Medicine, Gainesville, FL 32610; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cynthia L. Tannahill
*Department of Surgery, University of Florida College of Medicine, Gainesville, FL 32610; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Frances R. Bahjat
*Department of Surgery, University of Florida College of Medicine, Gainesville, FL 32610; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Caroline Oberholzer
*Department of Surgery, University of Florida College of Medicine, Gainesville, FL 32610; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andreas Oberholzer
*Department of Surgery, University of Florida College of Medicine, Gainesville, FL 32610; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Drake LaFace
†Canji, Incorporated, San Diego, CA 92121
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Beth Hutchins
†Canji, Incorporated, San Diego, CA 92121
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shufen Wen
†Canji, Incorporated, San Diego, CA 92121
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jeremy Shinoda
†Canji, Incorporated, San Diego, CA 92121
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Edward M. Copeland III
*Department of Surgery, University of Florida College of Medicine, Gainesville, FL 32610; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lyle L. Moldawer
*Department of Surgery, University of Florida College of Medicine, Gainesville, FL 32610; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Abstract

Adenovirus (Ad) gene therapy has been proposed as a drug-delivery system for the targeted administration of protein-based therapies, including growth factors and biological response modifiers. However, inflammation associated with Ad transduction has raised concern about its safety and efficacy in acute inflammatory diseases. In the present report, intratracheal and i.v. administration of a first-generation adenoviral recombinant (E1,E3 deleted) either containing an empty cassette or expressing the anti-inflammatory cytokines viral or human IL-10 (IL-10) was administered to mice subjected to zymosan-induced multisystem organ failure or to acute necrotizing pancreatitis. Pretreatment of mice with the intratracheal instillation of Ad expressing human IL-10 or viral IL-10 reduced weight loss, attenuated the proinflammatory cytokine response, and reduced mortality in the zymosan-induced model, whereas pretreatment with a control adenoviral recombinant did not significantly exacerbate the response. Pretreatment of mice with pancreatitis using adenoviral vectors expressing IL-10 significantly reduced the degree of pancreatic and liver injury and liver inflammation when administered systemically, but not intratracheally. We conclude that adenoviral vectors can be administered prophylactically in acute inflammatory syndromes, and expression of the anti-inflammatory protein IL-10 can be used to suppress the underlying inflammatory process.

  • Received February 15, 2001.
  • Accepted May 8, 2001.
  • Copyright © 2001 by The American Association of Immunologists
View Full Text
PreviousNext
Back to top

In this issue

The Journal of Immunology: 167 (2)
The Journal of Immunology
Vol. 167, Issue 2
15 Jul 2001
  • Table of Contents
  • About the Cover
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about The Journal of Immunology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Adenoviral Delivery of Human and Viral IL-10 in Murine Sepsis
(Your Name) has forwarded a page to you from The Journal of Immunology
(Your Name) thought you would like to see this page from the The Journal of Immunology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Adenoviral Delivery of Human and Viral IL-10 in Murine Sepsis
Rebecca M. Minter, Maria A. Ferry, Michelle E. Murday, Cynthia L. Tannahill, Frances R. Bahjat, Caroline Oberholzer, Andreas Oberholzer, Drake LaFace, Beth Hutchins, Shufen Wen, Jeremy Shinoda, Edward M. Copeland, Lyle L. Moldawer
The Journal of Immunology July 15, 2001, 167 (2) 1053-1059; DOI: 10.4049/jimmunol.167.2.1053

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Adenoviral Delivery of Human and Viral IL-10 in Murine Sepsis
Rebecca M. Minter, Maria A. Ferry, Michelle E. Murday, Cynthia L. Tannahill, Frances R. Bahjat, Caroline Oberholzer, Andreas Oberholzer, Drake LaFace, Beth Hutchins, Shufen Wen, Jeremy Shinoda, Edward M. Copeland, Lyle L. Moldawer
The Journal of Immunology July 15, 2001, 167 (2) 1053-1059; DOI: 10.4049/jimmunol.167.2.1053
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Antigen presentation by dendritic cells in the aortic wall triggers T helper immune responses in atherosclerosis (54.16)
  • Eph receptors are involved in the pro-inflammatory response following spinal cord injury (54.21)
  • Liver sinusoidal endothelial cells undergo apoptosis during sepsis, leading to organ dysfunction. (54.13)
Show more Inflammation

Similar Articles

Navigate

  • Home
  • Current Issue
  • Next in The JI
  • Archive
  • Brief Reviews
  • Pillars of Immunology
  • Translating Immunology

For Authors

  • Submit a Manuscript
  • Instructions for Authors
  • About the Journal
  • Journal Policies
  • Editors

General Information

  • Advertisers
  • Subscribers
  • Rights and Permissions
  • Accessibility Statement
  • Public Access
  • Privacy Policy
  • Disclaimer

Journal Services

  • Email Alerts
  • RSS Feeds
  • ImmunoCasts
  • Twitter

Copyright © 2021 by The American Association of Immunologists, Inc.

Print ISSN 0022-1767        Online ISSN 1550-6606