Article Figures & Data
Figures
- FIGURE 1.
E1A-transfected, but not Ad5-infected, human tumor cells are sensitive to TRAIL-induced lysis. A2058 and A2058-E1A are parental and E1A-transfected, human melanoma cells, respectively. H4 and H4-E1A are parental and E1A-transfected fibrosarcoma cells. A, TRAIL-induced lysis of A2058 (○), Ad5-infected-A2058 (•), or A2058-E1A (▪) cells. B, TRAIL-induced killing of H4 (○), Ad5-infected H4 (•), or H4-E1A (▪) cells.
- FIGURE 2.
Expression of E1A in Ad5-infected compared with E1A-transfected human tumor cells. A2058 cells were infected with Ad5 for 16 h and the levels of E1A oncoprotein expression were determined by Western analysis (see Materials and Methods). A2058-E1A is an E1A-transfected A2058 cell line.
- FIGURE 3.
Ad5-infection inhibits TRAIL-dependent killing of E1A-transfected cells. Parental and E1A-transfected A2058 cells were mock or Ad5-infected for 16 h and their sensitivity to TRAIL-induced killing was determined in a 16-h cytolysis assay. TRAIL-induced lysis of A2058 (○), Ad5-infected A2058-E1A (•), or uninfected A2058-E1A cells (▪).
- FIGURE 4.
E1B and E3 gene products inhibit TRAIL-dependent killing. H5dl250 and H5dl327 are Ad5-mutants that delete the E1B-19K and E3 open reading frames, respectively. Tumor cell lines were mock-, H5dl250-, or H5-dl327-infected for 16 h and their sensitivity to TRAIL-induced killing was determined in 16-h cytolysis assays. A, TRAIL-induced killing of A2058 (○), A2058-E1A (•), H5dl327- (▪), or H5d/250-infected (□) A2058 cells. B, TRAIL-induced killing of A2058 (○), H5dl327-infected (▪), or unifected-A2058-E1A cells (•).
In this issue
