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Human Galectin-3 Is a Novel Chemoattractant for Monocytes and Macrophages

Hideki Sano, Daniel K. Hsu, Lan Yu, John R. Apgar, Ichiro Kuwabara, Tohru Yamanaka, Mitsuomi Hirashima and Fu-Tong Liu
J Immunol August 15, 2000, 165 (4) 2156-2164; DOI: https://doi.org/10.4049/jimmunol.165.4.2156
Hideki Sano
*Division of Allergy, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121;
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Daniel K. Hsu
*Division of Allergy, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121;
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Lan Yu
*Division of Allergy, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121;
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John R. Apgar
†Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037;
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Ichiro Kuwabara
*Division of Allergy, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121;
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Tohru Yamanaka
‡First Department of Internal Medicine, Kumamoto University School of Medicine, Kumamoto, Japan; and
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Mitsuomi Hirashima
§Department of Immunology and Immunopathology, Kagawa Medical School, Kagawa, Japan
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Fu-Tong Liu
*Division of Allergy, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121;
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Abstract

Galectin-3 is a β-galactoside-binding protein implicated in diverse biological processes. We found that galectin-3 induced human monocyte migration in vitro in a dose-dependent manner, and it was chemotactic at high concentrations (1.0 μM) but chemokinetic at low concentrations (10–100 nM). Galectin-3-induced monocyte migration was inhibited by its specific mAb and was blocked by lactose and a C-terminal domain fragment of the protein, indicating that both the N-terminal and C-terminal domains of galectin-3 are involved in this activity. Pertussis toxin (PTX) almost completely blocked monocyte migration induced by high concentrations of galectin-3. Galectin-3 caused a Ca2+ influx in monocytes at high, but not low, concentrations, and both lactose and PTX inhibited this response. There was no cross-desensitization between galectin-3 and any of the monocyte-reactive chemokines examined, including monocyte chemotactic protein-1, macrophage inflammatory protein-1α, and stromal cell-derived factor-1α. Cultured human macrophages and alveolar macrophages also migrated toward galectin-3, but not monocyte chemotactic protein-1. Finally, galectin-3 was found to cause monocyte accumulation in vivo in mouse air pouches. These results indicate that galectin-3 is a novel chemoattractant for monocytes and macrophages and suggest that the effect is mediated at least in part through a PTX-sensitive (G protein-coupled) pathway.

  • Received January 11, 2000.
  • Accepted May 31, 2000.
  • Copyright © 2000 by The American Association of Immunologists
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The Journal of Immunology: 165 (4)
The Journal of Immunology
Vol. 165, Issue 4
15 Aug 2000
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Human Galectin-3 Is a Novel Chemoattractant for Monocytes and Macrophages
Hideki Sano, Daniel K. Hsu, Lan Yu, John R. Apgar, Ichiro Kuwabara, Tohru Yamanaka, Mitsuomi Hirashima, Fu-Tong Liu
The Journal of Immunology August 15, 2000, 165 (4) 2156-2164; DOI: 10.4049/jimmunol.165.4.2156

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Human Galectin-3 Is a Novel Chemoattractant for Monocytes and Macrophages
Hideki Sano, Daniel K. Hsu, Lan Yu, John R. Apgar, Ichiro Kuwabara, Tohru Yamanaka, Mitsuomi Hirashima, Fu-Tong Liu
The Journal of Immunology August 15, 2000, 165 (4) 2156-2164; DOI: 10.4049/jimmunol.165.4.2156
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