Article Figures & Data
Figures
- FIGURE 1.
Survival of TLR2-deficient mice (n = 10, ▪) and wild-type mice (n = 10, ○) after i.v. infection of 1 × 107 CFU of S. aureus. p < 0.03 in log rank test.
- FIGURE 2.
Numbers of live S. aureus in TLR2-deficient and wild-type mice after injection of bacteria. TLR2-deficient mice (n = 4 or 5) and wild-type mice (n = 4 or 5) were infected i.v. with 1 × 107 CFU (A) or 1 × 106 CFU (B) S. aureus. The blood and organs were collected 1 and 2 days (A) or 2 and 5 days (B) after injection, and bacterial counts were determined by culture on agar. Data are represented as the mean number of CFU ± SD. ∗, p < 0.05 in Mann-Whitney U test.
- FIGURE 3.
Survival of MyD88-deficient mice (n = 10, ○) and wild-type mice (n = 10, ▪) after i.v. infection of 1 × 107 CFU of S. aureus. p < 0.0003 in log rank test.
- FIGURE 4.
Numbers of live S. aureus in MyD88-deficient and wild-type mice after injection of bacteria. MyD88-deficient mice (n = 3 or 4) and wild-type mice (n = 3 or 4) were infected i.v. with 1 × 107 CFU (A) or 1 × 106 CFU (B) S. aureus. The blood and organs were collected 1 (A) or 2 days and 5 days (B) after injection, and bacterial counts were determined by culture on agar. Data are represented as the mean number of CFU ± SD. ∗, p < 0.05 in Mann-Whitney U test.
- FIGURE 5.
Production of TNF-α and IL-6 in response to heat-killed S. aureus in TLR2-, TLR4-, and MyD88-deficient macrophages. Peritoneal macrophages from wild-type, TLR2-, TLR4-, and MyD88-deficient mice were cultured in the presence of 1 × 107 CFU/ml of heat-killed S. aureus for 24 h. Concentrations of TNF-α (A) and IL-6 (B) in the culture supernatants were measured by ELISA. The results are representative of three independent experiments and are shown as the mean ± SEM of triplicate wells. ∗, p < 0.05 in Mann-Whitney U test. N.D., Not detected.
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