Article Figures & Data
Figures
- FIGURE 1.
C5a and antagonist-binding affinity to rat and human PMNs. Rat PMNs were isolated from whole blood and incubated with 50 pM 125I-C5a in the presence of either C5a or AcF-[OPdChaWR]. Both C5a and antagonist inhibited binding of 125I-C5a binding to isolated PMNs to give a relative binding affinity value. IC50 values were obtained from each experiment and the means were determined. Data are shown as a percentage of maximal binding of 125I-C5a (mean ± SEM, n = 3–4).
- FIGURE 2.
Inhibition of C5a- and LPS-induced neutropenia and TNF-α expression with a C5a receptor antagonist. Intravenous C5a (2 μg/kg) or LPS (1 mg/kg) resulted in a rapid and transient neutropenia (A and B) and a significant increase in detectable circulating TNF-α (C and D). Pretreatment of rats with i.v. C5a receptor antagonist (1 mg/kg) inhibited neutropenia and elevated cytokine expression. Data are shown as means ± SEM (n = 3–6).
- FIGURE 3.
Reverse-passive Arthus-induced vascular leakage and cell migration. Initiation of a peritoneal Arthus reaction resulted in a significant increase in optical density (A), total cell number (B), and total PMN number (C) in the peritoneal lavage fluid 4 h after induction of immune complex formation. Data are shown for Ab, Ag, and antagonist control rats, Arthus-treated rats, and rats pretreated with antagonist (1 mg/kg i.v.) before initiation of a peritoneal Arthus reaction. The peritoneal cavity was lavaged 4 h after Arthus reaction induction, and total cell number was counted, the OD of the supernatant was recorded, and total PMN number was determined from a differential cell count. Data are shown as means ± SEM (n = 3–5). ∗, p ≤ 0.05 vs Ab control.
- FIGURE 4.
Time course of reverse-passive Arthus-induced elevated serum cytokine levels. Initiation of a peritoneal Arthus reaction resulted in significantly elevated serum TNF-α (A) and IL-6 (B) concentrations in the serum which had resolved within 4 h. Data are shown for Ab, Ag, and antagonist control rats, Arthus-treated rats, and rats pretreated with antagonist (1 mg/kg i.v.) before initiation of a peritoneal Arthus reaction. Serum was collected periodically over the 4-h treatment period, and serum cytokine levels were measured using ELISA. Data are shown as means ± SEM (n = 3–5). ∗, p ≤ 0.05 vs Ab control.
- FIGURE 5.
Reverse-passive Arthus-induced elevation of peritoneal cytokine levels. Initiation of a peritoneal Arthus reaction resulted in significantly elevated serum TNF-α (A) and IL-6 (B) concentrations in the peritoneal lavage fluid 4 h after induction of immune complex formation. Data are shown for the Ab, Ag, and antagonist control rats, Arthus-treated rats, and rats pretreated with antagonist (1 mg/kg i.v.) before initiation of a peritoneal Arthus reaction. The peritoneal cavity was lavaged 4 h after Arthus reaction induction, and cytokine levels were measured using ELISA. Data are shown as means ± SEM (n = 3–5). ∗, p ≤ 0.05 vs Ab control.
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