Article Figures & Data
Figures
- FIGURE 1.
Kinetics of 4-1BB expression on in vivo activated T cells. B10.A mice were injected with 0.30 μg of SEA. At various times after SEA injection, LN T cells were purified. These cells were stained for CD4 Vβ3 4-1BB (left panel) and CD8 Vβ3 4-1BB (right panel) and analyzed by flow cytometry (solid lines). 4-1BB expression on T cells from an uninjected normal mouse (dotted lines) is shown. This is one experiment similar to three others.
- FIGURE 2.
Time course of SEA-stimulated CD4 T cell deletion with and without 4-1BB activation. B10.A mice were coinjected with 0.10 mg of anti-4-1BB mAb 3H3 (○) or 0.1 mg rat IgG (□) and 0.30 μg of SEA. Splenic T cells were purified on days 2, 5, 7, 12, and 21 after SEA injection. T cells were stained for CD4 Vβ3 and analyzed by flow cytometry. Each point represents the mean percentages (A) and numbers (B) ± SEM from six mice over three separate experiments combined.
- FIGURE 3.
Time course of SEA-stimulated CD8 T cell deletion with and without 4-1BB activation. These data are from the experiments described in Fig. 2, except the cells were stained (A) and enumerated (B) for CD8 Vβ3. Data from LN were similar (not shown).
- FIGURE 4.
Survival of SEA-stimulated and unstimulated CD8 T cells in vitro with and without 4-1BB activation. Three female B10.A mice were set up as follows: one was uninjected (□); one was injected with 0.20 mg of anti-4-1BB mAb 3H3 and 0.30 μg SEA (○); and one was injected with 0.20 mg of rat IgG and 0.30 μg SEA (⋄). LN T cells were purified 2 days after injections and cultured at 2 × 106 cells per well in a 96-well plate. T cells were stained and enumerated for CD8 Vβ14 (data not shown) and CD8 Vβ3 expression at 0, 8, 12, and 16 h in culture. Each data point represents the mean numbers ± SEM from triplicate samples for each injection regimen. These data are comparable to three other experiments.
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