Cutting Edge: IL-4-Independent Induction of Airway Hyperresponsiveness by Th2, But Not Th1, Cells

Lauren Cohn, Jeffrey S. Tepper and Kim Bottomly
J Immunol October 15, 1998, 161 (8) 3813-3816;

Article Figures & Data

Figures

  • FIGURE 1.
    FIGURE 1.

    A, Cytokine production by Th1 or Th2 cells. At the time of transfer into recipient mice, in vitro-generated Th1 or Th2 cells were cultured with APCs and pOVA323–339. Supernatants were collected after 48 h, and cytokine ELISAs were performed. B, BAL cell recovery in mice after transfer of cells, Th1, Th2, or none (−), and exposure to inhaled OVA. Differential counts were performed on cytospins of cells recovered from BAL from individual mice. M, macrophages; L, lymphocytes; N, neutrophils; E, eosinophils. Mean cell counts are shown (n = 5 mice per group). One experiment is shown and is representative of three experiments.

  • FIGURE 2.
    FIGURE 2.

    Airway reactivity to ACh after transfer of cells and exposure to inhaled OVA. The change in pulmonary resistance from baseline was determined in response to increasing doses of i.v. Ach in mice after transfer of cells, Th1, Th2, or none (−), and exposure to inhaled OVA (n = 3–5 mice per group); p < 0.0008 Th2/OVA vsTh1/OVA; p < 0.04; Th2/OVA vs −/OVA in three experiments.

  • FIGURE 3.
    FIGURE 3.

    A, Cytokine production by Th2 or IL-4−/− Th2 cells. At the time of transfer into recipient mice, in vitro generated Th2 or IL-4−/− Th2 cells were cultured with APCs and pOVA323–339. Supernatants were collected after 48 h, and cytokine ELISAs were performed. B, BAL cell recovery in mice after transfer of cells, Th2, IL-4−/− Th2 (IL-4−/−), or none (−), and exposure to inhaled OVA. Differential counts were performed on cytospins of cells recovered from BAL from individual mice. M, macrophages; L, lymphocytes; N, neutrophils; E, eosinophils. Mean cell counts are shown (n = 5 mice per group). One experiment is shown and is representative of three experiments.

  • FIGURE 4.
    FIGURE 4.

    Airway reactivity to ACh induced by Th2 cells in the absence of IL-4. The change in pulmonary resistance from baseline was determined in response to increasing doses of i.v. ACh in BALB/c recipient mice after transfer of cells, Th2, IL-4−/− Th2 (IL-4−/−), or none (−), and exposure to inhaled OVA (n = 5–6 mice per group); p < 0.01 Th2/OVA vs −/OVA; p < 0.04 IL-4−/− Th2/OVA vs −/OVA. One experiment is shown and is representative of two experiments.

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The Journal of Immunology
Vol. 161, Issue 8
15 Oct 1998
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