Article Figures & Data
Figures
- FIGURE 1.
Binding of influenza-specific S-IgA and pIgA by anti-rat SC and anti-mouse α antibodies. Purified monoclonal pIgA (open symbols) or S-IgA (closed symbols) was assayed for its ability to bind to influenza virus in an ELISA. Bound Abs were assayed for mouse α-chains (circles) or rat SC (squares) with the appropriate antisera. The dotted line represents the background level of anti-SC binding.
- FIGURE 2.
ELISA titers of pIgA, S-IgA, and mIgA anti-influenza mAbs. Purified S-IgA (closed squares), pIgA (open squares), and mIgA (closed circles) were tested simultaneously for their ability to bind to influenza virus in an ELISA. One ELISA binding unit of each preparation was calculated as the Ab dilution that gave an OD of 1 in this assay.
- FIGURE 3.
Hemagglutination inhibition by pIgA, S-IgA, and mIgA. PR8 influenza virus (2 HAU) was incubated for 30 min at room temperature with varying amounts of purified pIgA, S-IgA, or mIgA, then mixed with 0.5% CRBCs and incubated on ice until an HA pattern was apparent.
- FIGURE 4.
Neutralization of PR8 influenza virus by pIgA, S-IgA, and mIgA anti-H1 mAbs. Various dilutions of purified pIgA (open squares), S-IgA (closed squares), or mIgA (closed circles) were incubated on ice for 1 h with 102.5 TCID50 PR8 influenza virus, then 10-fold serial dilutions of the neutralization mixture were assayed on MDCK cells using the standard neutralization protocol. Fractionated normal rat bile and ZF11-15 IgA Ab, which were included as controls, did not neutralize virus.
- FIGURE 5.
Effect of trypsin preincubation. S-IgA or pIgA (0.1, 1, or 10 EBU) or mIgA (1, 10, or 100 EBU) was preincubated overnight at 34°C in the presence or the absence of 0.0002% trypsin, then incubated on ice for 1 h with 102.5 TCID50 PR8 influenza virus. Tenfold serial dilutions of the neutralization mixture were assayed on MDCK cells using the standard neutralization protocol.
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