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TCR-beta repertoire development in the mouse embryo.

H Ema, A Cumano and P Kourilsky
J Immunol November 1, 1997, 159 (9) 4227-4232;
H Ema
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A Cumano
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P Kourilsky
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Abstract

We studied the TCR beta-chain repertoire in thymocytes along embryonic development. At day 14 of gestation, complete V-D-J rearrangements of the TCR-beta locus were detected at the messenger RNA level, and a total of 56 different rearrangements were observed in one thymus. At this stage, thymocytes showed preferential usage of TCR-betaV1, TCR-betaV2, and TCR-betaV8 together with TCR-betaJ1S1, -J1S2, and -J2S7 segments. This frequent usage of V and J segments was similar to the usage seen in the adult thymus. Assuming that day 14 thymocytes were not subjected to specificity selection, we conclude that the expression of TCR-betaV and J segments is biased from the beginning of T cell repertoire creation. The first detectable transcripts of rearranged TCR-betas were different among individual fetuses and constituted a random representation of the ones seen at later stages of thymic ontogeny. The diversity of TCR-beta rearrangements increased with time and became indistinguishable from that of the adult by day 16. The first rearranged TCR-alphas were expressed at day 17 and their diversity increased thereafter. We conclude that the diversification of the beta-chain repertoire takes place in 3 days, before the alpha-chain rearrangement begins in transition from CD4/CD8 double negative to double positive cells.

  • Copyright © 1997 by American Association of Immunologists

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The Journal of Immunology
Vol. 159, Issue 9
1 Nov 1997
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TCR-beta repertoire development in the mouse embryo.
H Ema, A Cumano, P Kourilsky
The Journal of Immunology November 1, 1997, 159 (9) 4227-4232;

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TCR-beta repertoire development in the mouse embryo.
H Ema, A Cumano, P Kourilsky
The Journal of Immunology November 1, 1997, 159 (9) 4227-4232;
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Print ISSN 0022-1767        Online ISSN 1550-6606