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Evidence that the two C1q binding membrane proteins, gC1q-R and cC1q-R, associate to form a complex.

B Ghebrehiwet, P D Lu, W Zhang, S A Keilbaugh, L E Leigh, P Eggleton, K B Reid and E I Peerschke
J Immunol August 1, 1997, 159 (3) 1429-1436;
B Ghebrehiwet
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P D Lu
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W Zhang
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S A Keilbaugh
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L E Leigh
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P Eggleton
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K B Reid
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E I Peerschke
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Abstract

Two types of widely coexpressed, highly acidic, cell membrane binding proteins that display preferential domain specificity for C1q have been described: a 60-kDa calreticulin homologue, designated cC1q-R, that binds to the collagen-like "stalk" and a 33-kDa glycoprotein with affinity for the globular "heads" (gC1q-R). Although the two molecules are known to be coexpressed on all cell types examined to date and often coelute during purification, there is no direct evidence showing that they associate with each other either on the membrane or when examined in a purified system. In this report we present the first evidence that 1) biotinylated cC1q-R binds to recombinant as well as native gC1q-R, as assessed by solid phase ELISA; 2) binding sites for cC1q-R are located within N-terminal residues 76 through 93 of the mature form of gC1q-R and within residues 204 through 218; 3) this interaction is inhibited by two mAbs, 60.11 and 46.23, that recognize primarily epitopes within the N terminus of gC1q-R corresponding to residues 74 through 96 and by mAb 74.5.2 that recognizes epitopes within residues 204 through 218; and 4) biotinylated cC1q-R binds to microtiter-fixed Raji and K562 cells, and this interaction is inhibited by mAb 60.11. Furthermore, coimmunoprecipitation analysis of Raji cell membranes with anti-gC1q-R mAbs showed the presence of cC1q-R in addition to gC1q-R. Taken together, the evidence suggests that cC1q-R is able to form a complex with gC1q-R and may associate with gC1q-R on the cell surface.

  • Copyright © 1997 by American Association of Immunologists

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The Journal of Immunology
Vol. 159, Issue 3
1 Aug 1997
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Evidence that the two C1q binding membrane proteins, gC1q-R and cC1q-R, associate to form a complex.
B Ghebrehiwet, P D Lu, W Zhang, S A Keilbaugh, L E Leigh, P Eggleton, K B Reid, E I Peerschke
The Journal of Immunology August 1, 1997, 159 (3) 1429-1436;

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Evidence that the two C1q binding membrane proteins, gC1q-R and cC1q-R, associate to form a complex.
B Ghebrehiwet, P D Lu, W Zhang, S A Keilbaugh, L E Leigh, P Eggleton, K B Reid, E I Peerschke
The Journal of Immunology August 1, 1997, 159 (3) 1429-1436;
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Print ISSN 0022-1767        Online ISSN 1550-6606