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MUC18/MCAM (CD146), an activation antigen of human T lymphocytes.

W F Pickl, O Majdic, G F Fischer, P Petzelbauer, I Faé, M Waclavicek, J Stöckl, C Scheinecker, T Vidicki, H Aschauer, J P Johnson and W Knapp
J Immunol March 1, 1997, 158 (5) 2107-2115;
W F Pickl
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O Majdic
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G F Fischer
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P Petzelbauer
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I Faé
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M Waclavicek
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J Stöckl
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C Scheinecker
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T Vidicki
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H Aschauer
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J P Johnson
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W Knapp
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Abstract

Extravasation and tissue infiltration of leukocytes and metastatic tumor cells require the regulated expression and function of adhesive and pro-proteolytic surface molecules. We demonstrate here that human T cells, upon activation, neo-express the melanoma metastasis-associated surface molecule MUC18/melanoma cell adhesion molecule (MCAM). Expression of MUC18/MCAM (CD146) on T cells could be identified with two mAbs (541-10B2 and 541-2E5) obtained after immunization with HUT102 T cells and found to react with activated T cells. The specificity of our mAbs for MUC18/MCAM (CD146) was revealed by 1) definition of the appropriate molecular mass of approximately 110 kDa unreduced and 120 kDa reduced, 2) reactivity of mAbs with MUC18/MCAM (CD146) cDNA-transfected mouse L cells, 3) conclusive crosswise immunoblotting experiments with MUC18/MCAM (CD146)-specific mAbs, and 4) N-terminal amino acid sequencing of precipitated protein. In vitro activation by PHA caused neo-expression of MUC18/MCAM (CD146) on peripheral blood T cells within 1 day of stimulation, reaching a maximum on day 3. In vivo expression of MUC18/MCAM (CD146) was confirmed on CD3+ T cells infiltrating delayed-type hypersensitivity lesions of the skin, on synovial fluid T cells of rheumatoid arthritis patients, and on distinct T leukemia cells. MUC18/MCAM (CD146) cell surface expression on activated T cells is mirrored by the presence of specific mRNA. Leukocytes of healthy donors do not show significant MUC18/MCAM (CD146) expression. The finding that MUC18/MCAM (CD146) is also expressed on activated T cells might suggest that this adhesion molecule is involved in the extravasation and/or homing of activated T cells.

  • Copyright © 1997 by American Association of Immunologists

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The Journal of Immunology
Vol. 158, Issue 5
1 Mar 1997
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MUC18/MCAM (CD146), an activation antigen of human T lymphocytes.
W F Pickl, O Majdic, G F Fischer, P Petzelbauer, I Faé, M Waclavicek, J Stöckl, C Scheinecker, T Vidicki, H Aschauer, J P Johnson, W Knapp
The Journal of Immunology March 1, 1997, 158 (5) 2107-2115;

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MUC18/MCAM (CD146), an activation antigen of human T lymphocytes.
W F Pickl, O Majdic, G F Fischer, P Petzelbauer, I Faé, M Waclavicek, J Stöckl, C Scheinecker, T Vidicki, H Aschauer, J P Johnson, W Knapp
The Journal of Immunology March 1, 1997, 158 (5) 2107-2115;
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Print ISSN 0022-1767        Online ISSN 1550-6606