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Phorbol ester and calcium ionophore can replace TCR signals that induce positive selection of CD4 T cells.

Y Takahama and H Nakauchi
J Immunol August 15, 1996, 157 (4) 1508-1513;
Y Takahama
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H Nakauchi
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Abstract

Positive selection of immature thymocytes is a developmental process in which TCR ligation by low avidity interaction induces the generation of mature T cells. However, biochemical signals that can induce positive selection have been unclear. By using TCR-alpha beta- mutant thymus cultures, the present study shows that direct stimulation of intracellular signals by PMA and calcium ionophore ionomycin can induce the generation of mature CD4+8- T cells, bypassing TCR-induced positive selection signals. Interestingly, the concentrations of phorbol ester that induced positive selection were more restricted than those that induced mature T cell activation. Moreover, the combination of phorbol ester and ionomycin restored the generation of CD4+8- T cells in class II MHC- thymus cultures, but did not induce the generation of CD4-8+ T cells in class I MHC- thymus cultures. These results identify that the combination of protein kinase C activation and calcium elevation is the biochemical signal that can induce positive selection of CD4+ T cells.

  • Copyright © 1996 by American Association of Immunologists

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The Journal of Immunology
Vol. 157, Issue 4
15 Aug 1996
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Phorbol ester and calcium ionophore can replace TCR signals that induce positive selection of CD4 T cells.
Y Takahama, H Nakauchi
The Journal of Immunology August 15, 1996, 157 (4) 1508-1513;

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Phorbol ester and calcium ionophore can replace TCR signals that induce positive selection of CD4 T cells.
Y Takahama, H Nakauchi
The Journal of Immunology August 15, 1996, 157 (4) 1508-1513;
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Print ISSN 0022-1767        Online ISSN 1550-6606