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Two Listeria monocytogenes CTL epitopes are processed from the same antigen with different efficiencies.

A J Sijts, A Neisig, J Neefjes and E G Pamer
J Immunol January 15, 1996, 156 (2) 683-692;
A J Sijts
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A Neisig
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J Neefjes
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E G Pamer
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Abstract

Listeria monocytogenes is an intracellular bacterium that elicits MHC class I-restricted CTL in infected mice. A major CTL specificity is the nonamer peptide p60 217-225, which is derived from the bacterial murein hydrolase p60 and presented by the H-2Kd MHC class I molecule. In this report, we identify a second H-2Kd presented peptide, encompassing residues 449-457 of p60, that is detected by L. monocytogenes-specific CTL. Both p60-derived CTL epitopes are good competitors for H-2Kd binding and TAP (transporter associated with Ag processing) transport. CTL clone WP11.12 lyses L. monocytogenes infected cells and recognizes naturally processed p60 449-457 acid eluted from L. monocytogenes-infected macrophages. Although both epitopes derive from the same Ag and bind the same allelic form of MHC class I, quantitative analysis reveals that the amount of p60 449-457 in infected cells is approximately 10-fold greater than the amount of p60 217-225. Shuffling p60 217-225 into position 449-457 decreases its processing efficiency, indicating that the large number of p60 449-457 epitopes cannot be entirely attributed to epitope-flanking sequences. Our findings indicate that CTL epitopes can be processed from Ags with markedly different kinetics and efficiencies. Intrinsic qualities of an epitope and its location within a protein influence the efficiency of Ag processing.

  • Copyright © 1996 by American Association of Immunologists
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The Journal of Immunology
Vol. 156, Issue 2
15 Jan 1996
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Two Listeria monocytogenes CTL epitopes are processed from the same antigen with different efficiencies.
A J Sijts, A Neisig, J Neefjes, E G Pamer
The Journal of Immunology January 15, 1996, 156 (2) 683-692;

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Two Listeria monocytogenes CTL epitopes are processed from the same antigen with different efficiencies.
A J Sijts, A Neisig, J Neefjes, E G Pamer
The Journal of Immunology January 15, 1996, 156 (2) 683-692;
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Print ISSN 0022-1767        Online ISSN 1550-6606