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Mouse surfactant protein-D. cDNA cloning, characterization, and gene localization to chromosome 14.

M Motwani, R A White, N Guo, L L Dowler, A I Tauber and K N Sastry
J Immunol December 15, 1995, 155 (12) 5671-5677;
M Motwani
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R A White
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N Guo
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L L Dowler
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A I Tauber
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K N Sastry
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Abstract

Surfactant protein-D (SP-D) is a collectin found associated with surfactant in the lung. SP-D has also been functionally characterized as an opsonin for diverse microorganisms and a chemoattractant for phagocytic cells. To determine the structure of mouse SP-D, we isolated and characterized clones from a B6/CBAF1J strain lung cDNA library using a PCR-derived genomic probe. The deduced sequence predicts a 19-amino acid signal sequence, a 25-amino acid long NH2 terminus with two cysteines, followed by an uninterrupted collagen domain with 59 Gly-X-Y repeats. Next, a short "neck" domain of 28 amino acids, with a potential to form trimeric alpha-helical coiled coil is found ending in a COOH-terminal 125-amino acid carbohydrate recognition domain. The mature mouse SP-D protein of 355 amino acids shows strong homology to rat (92% identity), human (76%), and bovine (72%) SP-D amino acid sequences. Northern blot and RT-PCR analysis revealed that the mouse SP-D gene is expressed predominantly in lung and, surprisingly, also in heart, stomach, and kidney but not in brain. In contrast, mouse surfactant protein-A (SP-A) mRNA expression was found to be restricted to lung. Human lung and stomach, but not heart or liver, were found to express SP-D mRNA, as determined by PCR. The mouse SP-D gene (Sftp4) has been localized to chromosome 14 (to a region syntenic to human chromosome 10), closely linked to the genes for other collagenous lectins, mannose-binding protein-A (MbI1), and SP-A (Sftp1).

  • Copyright © 1995 by American Association of Immunologists

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The Journal of Immunology
Vol. 155, Issue 12
15 Dec 1995
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Mouse surfactant protein-D. cDNA cloning, characterization, and gene localization to chromosome 14.
M Motwani, R A White, N Guo, L L Dowler, A I Tauber, K N Sastry
The Journal of Immunology December 15, 1995, 155 (12) 5671-5677;

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Mouse surfactant protein-D. cDNA cloning, characterization, and gene localization to chromosome 14.
M Motwani, R A White, N Guo, L L Dowler, A I Tauber, K N Sastry
The Journal of Immunology December 15, 1995, 155 (12) 5671-5677;
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Print ISSN 0022-1767        Online ISSN 1550-6606