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Salmonella typhimurium induces IFN-gamma production in murine splenocytes. Role of natural killer cells and macrophages.

L Ramarathinam, D W Niesel and G R Klimpel
J Immunol May 1, 1993, 150 (9) 3973-3981;
L Ramarathinam
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D W Niesel
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G R Klimpel
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Abstract

IFN-gamma is a cytokine known to play an important role in host defense against Salmonella typhimurium. The lymphoid cells required for in vitro production of IFN-gamma after S. typhimurium stimulation of mouse spleen cells was investigated. Spleen cells depleted of cells bearing NK1.1, asialo GM1, Thy 1.2, or CD5 resulted in a significant reduction in IFN-gamma production after stimulation with S. typhimurium. In contrast, Con A-induced IFN-gamma production was only slightly reduced after depletion of NK1.1- or asialo GM1-bearing cells. Spleen cells from SCID mice produced elevated levels of IFN-gamma after stimulation with S. typhimurium. IFN-gamma production by SCID spleen cells was dependent upon asialo GM1+ T cells, suggesting that NK cells were the cells producing IFN-gamma in response to S. typhimurium. Splenic adherent cells were required for optimal IFN-gamma production. However, direct contact between the adherent and nylon wool nonadherent (NWNA) cell populations was not essential. IFN-gamma production was observed when the adherent and NWNA cell populations were physically separated or when supernatant from S. typhimurium-stimulated adherent cells was added to NWNA cells. Optimal IFN-gamma production was dependent on the presence of TNF-alpha, inasmuch as addition of antibody to TNF-alpha to spleen cell or NWNA cell cultures significantly reduced IFN-gamma production. However, addition of rTNF-alpha did not induce IFN-gamma production by NWNA cells. These findings document the existence of a T-independent mechanism for early IFN-gamma production in response to S. typhimurium, and show that TNF-alpha is necessary but not sufficient for the production of IFN-gamma.

  • Copyright © 1993 by American Association of Immunologists

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The Journal of Immunology
Vol. 150, Issue 9
1 May 1993
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Salmonella typhimurium induces IFN-gamma production in murine splenocytes. Role of natural killer cells and macrophages.
L Ramarathinam, D W Niesel, G R Klimpel
The Journal of Immunology May 1, 1993, 150 (9) 3973-3981;

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Salmonella typhimurium induces IFN-gamma production in murine splenocytes. Role of natural killer cells and macrophages.
L Ramarathinam, D W Niesel, G R Klimpel
The Journal of Immunology May 1, 1993, 150 (9) 3973-3981;
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Print ISSN 0022-1767        Online ISSN 1550-6606