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Functional thrombin receptors on human T lymphoblastoid cells.

A Tordai, J W Fenton 2nd, T Andersen and E W Gelfand
J Immunol June 1, 1993, 150 (11) 4876-4886;
A Tordai
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J W Fenton 2nd
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T Andersen
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E W Gelfand
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Abstract

Human alpha-thrombin stimulated five different T lymphoblastoid cell lines to increase intracellular free Ca2+ concentrations, whereas five B cell lines did not similarly respond. The T cell intracellular free Ca2+ increased rapidly, plateaued within 10 to 20 s, and then declined without any measurable sustained intracellular free Ca2+ elevation. Liberation of inositol trisphosphate peaked within 60 s, and a rapid and sustained activation of protein kinase C was induced. The thrombin-specific inhibitor, hirudin, completely blocked the response to alpha-thrombin. Catalytically inactivated forms of alpha-thrombin failed to stimulate the T cells, whereas trypsin, gamma-thrombin (which lacks fibrinogen clotting activity), or the synthetic 14-amino acid thrombin receptor-activation peptide stimulated T cells, but required approximately 10-, approximately 15-, or approximately 100-fold higher concentrations, respectively, when compared to alpha-thrombin. Stimulation by thrombin receptor-activation peptide was desensitized by alpha-thrombin, and vice versa, but alpha-thrombin did not desensitize stimulation by mAb to the TCR/CD3 Ag. The presence of the receptor on T but not on B cells was confirmed by flow-cytometric analysis using a mAb against the human thrombin receptor. These findings demonstrate functional thrombin receptors on T lymphoblastoid cells that may be capable of activating the cells, independently of an ongoing immune response.

  • Copyright © 1993 by American Association of Immunologists

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The Journal of Immunology
Vol. 150, Issue 11
1 Jun 1993
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Functional thrombin receptors on human T lymphoblastoid cells.
A Tordai, J W Fenton, T Andersen, E W Gelfand
The Journal of Immunology June 1, 1993, 150 (11) 4876-4886;

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Functional thrombin receptors on human T lymphoblastoid cells.
A Tordai, J W Fenton, T Andersen, E W Gelfand
The Journal of Immunology June 1, 1993, 150 (11) 4876-4886;
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Print ISSN 0022-1767        Online ISSN 1550-6606