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Association of human lymph node homing receptor (Leu 8) with the TCR/CD3 complex.

Y Murakawa, Y Minami, W Strober and S P James
J Immunol March 15, 1992, 148 (6) 1771-1776;
Y Murakawa
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Y Minami
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W Strober
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S P James
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Abstract

Cross-linking of the human homologue of the murine MEL-14 lymph node homing receptor (Selectin-1, LECAM-1, Leu 8) on both T and B cells results in modification of cell function. To investigate this phenomenon, we performed studies to determine if the Leu 8 molecule influences T cell activation via the TCR/CD3 complex. In initial studies, we treated T cells with immobilized anti-CD3 (OKT3 mAb) in the presence or absence of immobilized Leu 8 mAb. We found that although Leu 8 mAb alone had no effect on T cell proliferation, this antibody markedly augmented immobilized OKT3 mAb-induced proliferation. In further studies, we immunoprecipitated surface radioiodinated T cell lysates with OKT3 and Leu 8 mAb to determine if molecules in the TCR/CD3 complex associate with Leu 8 molecules. Although Leu 8 mAb immunoprecipitated only a single protein of approximately 80 kDa from T cell lysates treated with Nonidet P-40 under reducing condition, it coimmunoprecipitated additional proteins of 48, 42, 28, 24, and 22 kDa from T cell lysates treated with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate. These additional proteins were identified as the alpha-, beta-, gamma-, delta-, and epsilon-chains of the TCR/CD3 complex by one-dimensional and two-dimensional diagonal SDS-PAGE. Densitometric scanning showed that, on average, 18% of the TCR/CD3 complex associates with Leu 8. In a final study, we showed by immunoblotting analysis using anti-zeta peptide antibody that Leu 8 mAb coimmunoprecipitates the zeta-chain of CD3. These results indicate that the human lymph node homing receptor homologue (Leu 8) participates in the activation of T cells, probably via its association with the TCR/CD3 complex.

  • Copyright © 1992 by American Association of Immunologists

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The Journal of Immunology
Vol. 148, Issue 6
15 Mar 1992
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Association of human lymph node homing receptor (Leu 8) with the TCR/CD3 complex.
Y Murakawa, Y Minami, W Strober, S P James
The Journal of Immunology March 15, 1992, 148 (6) 1771-1776;

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Association of human lymph node homing receptor (Leu 8) with the TCR/CD3 complex.
Y Murakawa, Y Minami, W Strober, S P James
The Journal of Immunology March 15, 1992, 148 (6) 1771-1776;
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Print ISSN 0022-1767        Online ISSN 1550-6606