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IL-12 receptor. II. Distribution and regulation of receptor expression.

B B Desai, P M Quinn, A G Wolitzky, P K Mongini, R Chizzonite and M K Gately
J Immunol May 15, 1992, 148 (10) 3125-3132;
B B Desai
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P M Quinn
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A G Wolitzky
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P K Mongini
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R Chizzonite
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M K Gately
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Abstract

IL-12 is a heterodimeric lymphokine that induces IFN-gamma production by resting PBMC, enhances the lytic activity of NK/lymphokine activated killer cells, and causes the proliferation of activated T cells and NK cells. In this report, we have investigated the expression of IL-12R on mitogen- and IL-2-activated PBMC or tonsillar lymphocytes as well as on a variety of cell lines. The results of radiolabeled IL-12-binding assays indicated that high affinity IL-12R are present on PBMC activated by various T cell mitogens or by IL-2. High affinity IL-12R were also found to be expressed constitutively on a transformed marmoset NK-like cell line HVS.SILVA 40. At the time of peak IL-12R expression, mitogen- or IL-2-activated cells displayed approximately 1000 to 9000 IL-12 binding sites/cell with an apparent Kd of 100 to 900 pM. Kinetic studies revealed that maximum expression of IL-12R occurred earlier on PHA-activated PBMC as compared with PBMC activated by IL-2, and that expression of IL-12R on these cells correlated with their ability to proliferate in response to IL-12. Although IL-2 could up-regulate IL-12R expression on resting PBMC, the ability of mitogen-activated PBMC to up-regulate IL-12R was found to be independent of IL-2. Analysis of IL-12R expression by flow cytometry revealed that receptors for IL-12 are present on activated T cells of both the CD4+ and CD8+ subsets and on activated CD56+ NK cells. In contrast, neither resting PBMC or tonsillar B cells nor tonsillar B cells activated by anti-IgM/Dx, anti-IgM/Dx + IL-2, or SAC + IL-2 displayed IL-12R detectable by flow cytometry or by the radiolabeled IL-12-binding assay. In summary, these results indicate that activation of T cells or NK cells results in up-regulation of IL-12R expression; on the other hand, B cell activation, at least under some circumstances, appears not to be associated with enhanced expression of IL-12R.

  • Copyright © 1992 by American Association of Immunologists

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The Journal of Immunology
Vol. 148, Issue 10
15 May 1992
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IL-12 receptor. II. Distribution and regulation of receptor expression.
B B Desai, P M Quinn, A G Wolitzky, P K Mongini, R Chizzonite, M K Gately
The Journal of Immunology May 15, 1992, 148 (10) 3125-3132;

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IL-12 receptor. II. Distribution and regulation of receptor expression.
B B Desai, P M Quinn, A G Wolitzky, P K Mongini, R Chizzonite, M K Gately
The Journal of Immunology May 15, 1992, 148 (10) 3125-3132;
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Print ISSN 0022-1767        Online ISSN 1550-6606