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Repertoire diversity of antibody response to bacterial antigens in aged mice. II. Phosphorylcholine-antibody in young and aged mice differ in both VH/VL gene repertoire and in specificity.

C Nicoletti, C Borghesi-Nicoletti, X H Yang, D H Schulze and J Cerny
J Immunol October 15, 1991, 147 (8) 2750-2755;
C Nicoletti
Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201.
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C Borghesi-Nicoletti
Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201.
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X H Yang
Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201.
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D H Schulze
Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201.
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J Cerny
Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201.
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Abstract

Aging of mice is accompanied by both quantitative and qualitative changes in antibody responses to phosphorylcholine (PC), an immunodominant epitope of Streptococcus pneumoniae R36a strain (Pn). In order to study these changes at the molecular level, we generated PC-specific hybridomas from young (3 to 4 mo) and aged (20 to 24 mo) mice of different strains after primary immunization with S. pneumoniae R36a strain. These mAb were tested for Ig VH and VL gene family utilization, idiotopic repertoire, and cross-reactivity with unrelated Ag. Hybridomas from young mice (BALB/c, C57BL/6, and D1.LP) uniformly expressed the VH-S107 and V kappa-22 genes as well as most idiotopes of the T15 family, which were identified with different anti-T15 mAb. In contrast, the PC-reactive mAb from aged mice were quite heterogeneous: only 2 out of 13 utilized VHS107, 1 of 13 used VH7183, and 3 of 13 used VHJ558 gene family. Moreover, none of these mAb used L chain encoded by V kappa 22(0/13), but surprisingly they frequently expressed some of the T15 idiotope. In addition, the PC-binding mAb from aged mice showed broad cross-reactivity with various mouse and foreign proteins, whereas the mAb from young mice did not. These results demonstrate the genetic shift in antibody response of aging mice to PC, which is accompanied by a change in the antibody specificity. Interestingly, the qualitative repertoire change appears to be unrelated to the magnitude of antibody response, for the aged BALB/c mice maintain a very high reactivity to PC.

  • Copyright © 1991 by American Association of Immunologists

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The Journal of Immunology
Vol. 147, Issue 8
15 Oct 1991
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Repertoire diversity of antibody response to bacterial antigens in aged mice. II. Phosphorylcholine-antibody in young and aged mice differ in both VH/VL gene repertoire and in specificity.
C Nicoletti, C Borghesi-Nicoletti, X H Yang, D H Schulze, J Cerny
The Journal of Immunology October 15, 1991, 147 (8) 2750-2755;

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Repertoire diversity of antibody response to bacterial antigens in aged mice. II. Phosphorylcholine-antibody in young and aged mice differ in both VH/VL gene repertoire and in specificity.
C Nicoletti, C Borghesi-Nicoletti, X H Yang, D H Schulze, J Cerny
The Journal of Immunology October 15, 1991, 147 (8) 2750-2755;
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Print ISSN 0022-1767        Online ISSN 1550-6606