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Nuclear transcription factors that bind to elements of the IL-2 promoter. Induction requirements in primary human T cells.

A Granelli-Piperno and P Nolan
J Immunol October 15, 1991, 147 (8) 2734-2739;
A Granelli-Piperno
Laboratory of Cellular Physiology and Immunology, Rockfeller University, New York, NY 10021.
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P Nolan
Laboratory of Cellular Physiology and Immunology, Rockfeller University, New York, NY 10021.
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Abstract

Prior studies have identified several elements that contribute to the activity of the IL-2 promoter in the stimulated T cell line, Jurkat. The sites and their corresponding nuclear binding factors include: NF-kappa B, AP-1, AP-3, OCT-1, and NF-AT. The latter "nuclear factor for activated T cells" likely contributes to the tissue specificity of IL-2 gene expression. Using electrophoretic mobility shift assays, we have studied these transcription factors in primary T cells from human blood to verify their presence in a physiologic setting and to identify the signals that stimulate factor activity. All factors are induced in the nuclei of T cells upon activation with mitogens but not with exogenous IL-2 growth factor. However, the signaling requirements and sensitivity to protein synthesis inhibitors differ considerably. Only the activities for NF-AT and AP-1 sites require two signals for optimal induction, i.e., PMA plus either lectin or antibody to the CD3 or CD28 surface molecules. Other factors are induced by lectin, antibody, and/or PMA alone. After appropriate stimulation, both NF-AT and AP-1 are peculiarly sensitive to the protein synthesis inhibitor anisomycin. Our data correlate the activity of NF-AT and AP-1 in gel shift assays with the two signals requirements for IL-2 gene expression.

  • Copyright © 1991 by American Association of Immunologists

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The Journal of Immunology
Vol. 147, Issue 8
15 Oct 1991
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Nuclear transcription factors that bind to elements of the IL-2 promoter. Induction requirements in primary human T cells.
A Granelli-Piperno, P Nolan
The Journal of Immunology October 15, 1991, 147 (8) 2734-2739;

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Nuclear transcription factors that bind to elements of the IL-2 promoter. Induction requirements in primary human T cells.
A Granelli-Piperno, P Nolan
The Journal of Immunology October 15, 1991, 147 (8) 2734-2739;
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Print ISSN 0022-1767        Online ISSN 1550-6606