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The structure and expression of genes encoding serologically undetected HLA-C locus antigens.

M Takiguchi, I Nishimura, H Hayashi, S Karakl, A Kariyone and K Kano
J Immunol August 15, 1989, 143 (4) 1372-1378;
M Takiguchi
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I Nishimura
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H Hayashi
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S Karakl
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A Kariyone
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K Kano
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Abstract

Approximately 20 to 50% individuals in every race are untypable by human alloantisera for at least one allele of HLA-C locus and the surface expression of HLA-C locus Ag in such an individual (HLA-C blank Ag) remains unknown. To investigate the structure and the surface expression of HLA-C blank Ag, two genes (Cb-1 and Cb-2) encoding HLA-C blank Ag were cloned and their primary structures were determined and compared with other HLA-C locus genes. The similarity of amino acids between Cb-1 and Cw1 was the highest among HLA-C locus genes previously published. Five amino acid substitutions between these molecules were shown to be located on the beta-strand of alpha 1 and alpha 2 domains, suggesting that they might change the conformational allodeterminants on the alpha-helical region of Cw1 which were recognized by antibodies. On the other hand, Cb-2 was the closest to Cw2.2. Six of nine amino acid substitutions between these molecules were observed on alpha 1 and alpha 2 domains, whereas three other substitutions were located on the leader peptide, the alpha 3 domain and the transmembrane. Two substitutions (residues 73 and 163) of the alpha-helical region of the alpha 1 and alpha 2 domains and one (residue 16) of exposed loop may make new allodeterminants which are not recognized by anti-Cw2 sera as well as other alloantisera. The surface expression of these genes was examined on transfected mouse L cells and human B cell line. Both gene products were expressed stably on the surface of these cells. These results suggest that HLA-C blank Ag are most probably expressed on cells in HLA-C blank individuals and that the primary structures of these Ag, which were not detectable by the available alloantisera, may be incapable of generating corresponding alloantibodies.

  • Copyright © 1989 by American Association of Immunologists

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The Journal of Immunology
Vol. 143, Issue 4
15 Aug 1989
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The structure and expression of genes encoding serologically undetected HLA-C locus antigens.
M Takiguchi, I Nishimura, H Hayashi, S Karakl, A Kariyone, K Kano
The Journal of Immunology August 15, 1989, 143 (4) 1372-1378;

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The structure and expression of genes encoding serologically undetected HLA-C locus antigens.
M Takiguchi, I Nishimura, H Hayashi, S Karakl, A Kariyone, K Kano
The Journal of Immunology August 15, 1989, 143 (4) 1372-1378;
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Print ISSN 0022-1767        Online ISSN 1550-6606