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Regulation of antigen presentation. II. Anti-Ig and IL-2 induce IL-1 production by murine splenic B cells.

C M Hawrylowicz, L M Duncan, R C Fuhlbrigge and E R Unanue
J Immunol May 15, 1989, 142 (10) 3361-3368;
C M Hawrylowicz
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L M Duncan
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R C Fuhlbrigge
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E R Unanue
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Abstract

Murine splenic B cells did not constitutively express IL-1 activity. After culture with anti-Ig and T cell-conditioned media and then fixation, B cells expressed membrane IL-1 and were able to stimulate growth of the IL-1-dependent T cell clone D10. Expression of membrane IL-1 required stimulation of B cells for 2 days before fixation. Significant IL-1 activity was detectable in freeze-thaw lysates of identical B cell preparations by 12 h. B cells also released IL-1 into the culture media. In situ hybridization studies by using probes to murine IL-1 alpha and IL-1 beta genes supported these observations. Thus, messenger RNA for IL-1 alpha and IL-1 beta rose in parallel, were detected between 6 and 24 h of culture, and declined to low levels by 30 h. Despite the presence of mRNA for IL-1 alpha and IL-1 beta, only IL-1 alpha had functional activity as determined by the use of a mAb to IL-1 alpha. IL-2 was found to be an essential component of the T cell-derived supernatant. Although IL-4 or TNF did not induce significant B cell IL-1 expression, they both caused a modest, but reproducible enhancement when added in combination with IL-2. IFN-gamma, by contrast, partially inhibited IL-1 induction.

  • Copyright © 1989 by American Association of Immunologists

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The Journal of Immunology
Vol. 142, Issue 10
15 May 1989
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Regulation of antigen presentation. II. Anti-Ig and IL-2 induce IL-1 production by murine splenic B cells.
C M Hawrylowicz, L M Duncan, R C Fuhlbrigge, E R Unanue
The Journal of Immunology May 15, 1989, 142 (10) 3361-3368;

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Regulation of antigen presentation. II. Anti-Ig and IL-2 induce IL-1 production by murine splenic B cells.
C M Hawrylowicz, L M Duncan, R C Fuhlbrigge, E R Unanue
The Journal of Immunology May 15, 1989, 142 (10) 3361-3368;
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Print ISSN 0022-1767        Online ISSN 1550-6606