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Expression of IL-1 receptors on human peripheral B cells.

Y Tanaka, F Shirakawa, S Oda, S Eto and U Yamashita
J Immunol January 1, 1989, 142 (1) 167-172;
Y Tanaka
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F Shirakawa
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S Oda
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S Eto
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U Yamashita
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Abstract

The expression of IL-1R on human peripheral B cells was analyzed by the binding assay with 125I-labeled human rIL-1 alpha and by the flow cytofluorometry by the use of FITC-conjugated IL-1 alpha. The proliferation and the differentiation of B cells stimulated with Staphylococcus aureus Cowan I (SAC) in the presence of T cell-derived factors were dependent on IL-1. By the binding experiment with 125I-labeled IL-1 alpha, B cells expressed only few IL-1R without any stimulations. When they were stimulated with SAC, IL-1R on B cells began to increase by only 1 h, reached the maximum level at 6 h. The binding of 125I-labeled IL-1 alpha to B cells was inhibited by the addition of either cold IL-1 alpha or IL-1 beta suggesting that IL-1R on B cells reactive for IL-1 alpha and IL-1 beta were identical. By Scatchard plot analysis, the existence of two classes of IL-1R on B cells was found. A major class of IL-1R (320 molecules/cell) has a lower affinity (Kd = 3.8 x 10(-10) M) and a minor class of IL-1R (70 molecules/cell) has a higher affinity (Kd = 4.4 x 10(-12) M). When B cells were stimulated with SAC, both lower and higher affinity IL-1R were increased to 1960 molecules/cell and 300 molecules/cell, respectively. Furthermore, IL-1R on B cells were also detected with FITC-conjugated IL-1 alpha by a flow cytofluorometer. Only 3 to 5% of B cells expressed IL-1R without any stimulations. When B cells were stimulated with SAC, IL-1R-positive B cells were increased to 20%. The addition of anti-class II antibodies inhibited B cell proliferation and differentiation induced with SAC, IL-1, and T cell-derived factors. Anti-class II antibodies also inhibited the number of IL-1R on B cells. These results suggest that the expression of IL-1R was induced as the initial stage of B cell activation and that class II Ag play an important role for the expression of IL-1R on B cells.

  • Copyright © 1989 by American Association of Immunologists

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The Journal of Immunology
Vol. 142, Issue 1
1 Jan 1989
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Expression of IL-1 receptors on human peripheral B cells.
Y Tanaka, F Shirakawa, S Oda, S Eto, U Yamashita
The Journal of Immunology January 1, 1989, 142 (1) 167-172;

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Expression of IL-1 receptors on human peripheral B cells.
Y Tanaka, F Shirakawa, S Oda, S Eto, U Yamashita
The Journal of Immunology January 1, 1989, 142 (1) 167-172;
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Print ISSN 0022-1767        Online ISSN 1550-6606