Killer cell systems of cynomolgus monkeys experimentally infected with HTLV-1.

Abstract

The cell-mediated killer activity in cynomolgus monkeys, which were infected 2.5 yr previously with HTLV-1, was examined. With HTLV-1-infected autologous lymphoid cells as targets, HTLV-1-specific killer cells were not detected among PBL cells of infected monkeys, but in monkeys in which specific memory cells were found. These memory cells were converted to active specific killer cells by stimulation with mytomycin C (MMC)-treated HTLV-1 infected autologous cells in vitro. Target blocking by anti-HTLV-1-related Ag suggested that the target molecules recognized by the specific killer cells may be virus envelope glycoprotein gp 68 and other cellular Ag induced by HTLV-1 infection. In addition to the specific killer cells, NK cells that could kill not only NK-sensitive target cells but also target lymphoid cells with HTLV-1 Ag on their surface, were found in these monkeys. In vitro stimulation caused enhancement of NK cell activity as well as induction of antigen specific killer cells. These findings suggest that Ag-specific killer cells may work together with NK cells to eliminate HTLV-1-bearing T cells in vivo.