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Cloning and high level expression of a chimeric antibody with specificity for human carcinoembryonic antigen.

C B Beidler, J R Ludwig, J Cardenas, J Phelps, C G Papworth, E Melcher, M Sierzega, L J Myers, B W Unger and M Fisher
J Immunol December 1, 1988, 141 (11) 4053-4060;
C B Beidler
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J R Ludwig
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J Cardenas
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J Phelps
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C G Papworth
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E Melcher
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M Sierzega
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L J Myers
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B W Unger
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M Fisher
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Abstract

A mouse/human chimeric antibody has been constructed by using variable light and variable heavy regions from a murine hybridoma specific for human carcinoembryonic antigen (CEA) (CEM231.6.7). These V regions were combined with kappa and gamma-1 constant region genes cloned from human lymphocytes. The chimeric constructs were sequentially electroporated into murine non-Ig-producing myeloma (P3.653) and hybridoma (SP2/0) cell. Significant differences were seen in expression levels between the two cell types. High levels of expression (24 to 32 micrograms/ml/10(6) cells) were seen with several of the anti-CEA SP2/0 transfectomas but not with the P3.653 cells. The SP2/0 transfectoma lines were adapted to serum-free, chemically defined media and grown in large scale fermentation cultures where they continued to secrete high levels of antibody. The chimeric antibodies remain reactive against human CEA with affinity constants comparable to that of the parental hybridoma antibody. High level expression will make practical the production of chimeric antibodies for in vivo therapeutic and diagnostic purposes.

  • Copyright © 1988 by American Association of Immunologists

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The Journal of Immunology
Vol. 141, Issue 11
1 Dec 1988
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Cloning and high level expression of a chimeric antibody with specificity for human carcinoembryonic antigen.
C B Beidler, J R Ludwig, J Cardenas, J Phelps, C G Papworth, E Melcher, M Sierzega, L J Myers, B W Unger, M Fisher
The Journal of Immunology December 1, 1988, 141 (11) 4053-4060;

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Cloning and high level expression of a chimeric antibody with specificity for human carcinoembryonic antigen.
C B Beidler, J R Ludwig, J Cardenas, J Phelps, C G Papworth, E Melcher, M Sierzega, L J Myers, B W Unger, M Fisher
The Journal of Immunology December 1, 1988, 141 (11) 4053-4060;
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Print ISSN 0022-1767        Online ISSN 1550-6606