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The generation of alloreactive cytotoxic T lymphocytes requires the expression of ecto-5'nucleotidase activity.

M Massaia, A Pileri, M Boccadoro, A Bianchi, A Palumbo and U Dianzani
J Immunol December 1, 1988, 141 (11) 3768-3775;
M Massaia
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A Pileri
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M Boccadoro
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A Bianchi
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A Palumbo
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U Dianzani
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Abstract

Ecto-5'-nucleotidase (ecto-5'NT) activity is highly expressed by CTL precursor cells. The aim of this work was to investigate whether ecto-5'NT is involved in their functional activation. The inhibition of ecto-5'NT activity by biochemical (alpha,beta-methyleneadenosine 5-diphosphate; AOPCP) or immunologic inhibitors (polyclonal anti-ecto-5'NT serum) suppressed the proliferative and cytotoxic activation of CTL against a lymphoblastic B cell line in primary one-way MLC. The kinetic analysis of suppression showed that AOPCP and anti-5'NT serum had different effects: AOPCP suppressed only the activation of the cytotoxic program whereas anti-5'NT serum suppressed also recognition and binding to the stimulatory/target cell. Inhibition of CTL generation was not a metabolic consequence of the purine salvage blockade. Incubation of AOPCP-treated MLC with hypoxanthine restored proliferation but not cytotoxicity. The ecto-5'NT inhibitors used had no general toxic effect on cell numbers or viability. AOPCP selectively affected CTL generation and displayed no activity against mitogen-induced proliferation, activation of Ts cells, and generation of IL-2-activated killer cells. These data indicate that ecto-5'NT has a critical role in the functional activation of alloreactive CTL.

  • Copyright © 1988 by American Association of Immunologists

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The Journal of Immunology
Vol. 141, Issue 11
1 Dec 1988
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The generation of alloreactive cytotoxic T lymphocytes requires the expression of ecto-5'nucleotidase activity.
M Massaia, A Pileri, M Boccadoro, A Bianchi, A Palumbo, U Dianzani
The Journal of Immunology December 1, 1988, 141 (11) 3768-3775;

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The generation of alloreactive cytotoxic T lymphocytes requires the expression of ecto-5'nucleotidase activity.
M Massaia, A Pileri, M Boccadoro, A Bianchi, A Palumbo, U Dianzani
The Journal of Immunology December 1, 1988, 141 (11) 3768-3775;
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