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Human monocytes generate basophil histamine-releasing activities.

E S Schulman, M C McGettigan, T J Post, R J Vigderman and S S Shapiro
J Immunol April 1, 1988, 140 (7) 2369-2375;
E S Schulman
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M C McGettigan
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T J Post
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R J Vigderman
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S S Shapiro
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Abstract

Human peripheral blood monocytes generated activities during 24-h culture that were capable of triggering histamine release from 17 of 18 human basophil donors. Monocytes and their in vitro transformed macrophages continued to elaborate these basophil histamine-releasing activities for at least 3 wk in culture. In the 18 basophil donors tested, maximum histamine release induced by monocyte supernatants was 33.8 +/- 5.9% (mean +/- SEM) of total basophil histamine content; optimum anti-IgE-induced release was 38.8 +/- 6.2%. Basophil histamine release in response to monocyte activities was optimal at 37 degrees C and at calcium concentrations of 2 to 5 mM. Release was greater than 90% complete 1 min after challenge and was inhibited by anti-allergic drugs. The mechanism of release appeared to be independent of IgE binding. Gel filtration of supernatants derived from both day 1 (monocyte stage) and day 14 (macrophage stage) cultures demonstrated activity peaks with approximate m.w. of 12,000 and 30,000. In contrast to the marked responsiveness of basophils, only 2 of 10 human lung mast cell preparations responded; release in those preparations was low: 3% and 13% histamine release, respectively. Thus, monocytes produce potent histamine-releasing activities with differential actions on basophils and mast cells.

  • Copyright © 1988 by American Association of Immunologists

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The Journal of Immunology
Vol. 140, Issue 7
1 Apr 1988
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Human monocytes generate basophil histamine-releasing activities.
E S Schulman, M C McGettigan, T J Post, R J Vigderman, S S Shapiro
The Journal of Immunology April 1, 1988, 140 (7) 2369-2375;

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Human monocytes generate basophil histamine-releasing activities.
E S Schulman, M C McGettigan, T J Post, R J Vigderman, S S Shapiro
The Journal of Immunology April 1, 1988, 140 (7) 2369-2375;
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Print ISSN 0022-1767        Online ISSN 1550-6606