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Ig isotypes produced by EBV-transformed B cells as a function of age and tissue distribution.

T Miyawaki, H Kubagawa, J L Butler and M D Cooper
J Immunol June 1, 1988, 140 (11) 3887-3892;
T Miyawaki
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H Kubagawa
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J L Butler
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M D Cooper
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Abstract

EBV can transform human B cells giving rise to lymphoblastoid cell lines that produce and secrete Ig. Herein B cells from various tissues of newborns and adults were transformed by EBV and their Ig products were analyzed with isotype-specific mAb. Although IgG- and IgA-bearing B cells were present in the newborn, EBV transformed IgM-producing cells almost exclusively in both newborn blood and breast milk. IgM-secreting cells were derived from IgM+ B cells and IgM- pre-B cells present in neonatal blood, but only from IgM+ cells in adult blood. Whereas in adults most EBV-transformed cells produced IgM, producers of IgG and of IgA were present in frequencies that varied according to the tissue source. Precursors of IgG-producing cells were relatively abundant in blood, spleen, and tonsil, and relatively infrequent in bone marrow and appendix. EBV-inducible IgA producers were relatively concentrated in the appendix and to a lesser extent in tonsils and blood. Differences in the subclass composition of EBV-transformed populations of IgG- and IgA-producers were also observed for the various adult lymphoid tissues. IgG1-producing cells predominated in most tissues, and precursors of IgG2 were largely confined to the circulation. Whereas IgA1-producing cells were predominant in all tissues, a marked enrichment in IgA2-producers was observed in the appendix. These results indicate a remarkable heterogeneity in the isotype distribution pattern of EBV-transformable B cells that is determined both by developmental age and tissue localization. We propose that EBV selectively transforms primed B cells, the isotype commitment of which varies according to tissue origin and age.

  • Copyright © 1988 by American Association of Immunologists

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The Journal of Immunology
Vol. 140, Issue 11
1 Jun 1988
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Ig isotypes produced by EBV-transformed B cells as a function of age and tissue distribution.
T Miyawaki, H Kubagawa, J L Butler, M D Cooper
The Journal of Immunology June 1, 1988, 140 (11) 3887-3892;

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Ig isotypes produced by EBV-transformed B cells as a function of age and tissue distribution.
T Miyawaki, H Kubagawa, J L Butler, M D Cooper
The Journal of Immunology June 1, 1988, 140 (11) 3887-3892;
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Print ISSN 0022-1767        Online ISSN 1550-6606