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The glycine-alanine repeating region is the major epitope of the Epstein-Barr nuclear antigen-1 (EBNA-1).

H Rumpold, G H Rhodes, P L Bloch, D A Carson and J H Vaughan
J Immunol January 15, 1987, 138 (2) 593-599;
H Rumpold
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G H Rhodes
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P L Bloch
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D A Carson
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J H Vaughan
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Abstract

The Epstein-Barr nuclear antigen-1 (EBNA-1) is a protein containing a large glycine-alanine repeat that has been shown to be antigenic. Antibodies to EBNA-1 can be detected by means of immunoblotting. Preincubation of antisera with purified EBNA-1 protein inhibits the binding of IgG antibodies in this system, indicating that those epitopes detected by immunoblots are also accessible on the native molecule. A number of synthetic peptides the sequences of which were derived from the glycine-alanine repeating region of EBNA-1 and from regions adjacent to it also inhibited antibody binding to EBNA-1. These showed, however, a 1000-fold variation in their inhibitory activities. Peptides containing only glycine and alanine were the most effective inhibitors. The anti-EBNA-1 antibodies did not react with several other peptides representing sequences from unrelated proteins. At saturating concentrations of peptide 85 to 100% of anti-EBNA-1, antibody binding was inhibited in all sera tested with one exception. Similar results are obtained when antibody binding is assayed by an enzyme immunosorbent assay by using partially purified EBNA-1 to coat the plates. Thus the glycine-alanine region, either through its primary structure or through conformations assumed by this region, forms the major epitope(s) of the EBNA-1 molecule.

  • Copyright © 1987 by American Association of Immunologists

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The Journal of Immunology
Vol. 138, Issue 2
15 Jan 1987
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The glycine-alanine repeating region is the major epitope of the Epstein-Barr nuclear antigen-1 (EBNA-1).
H Rumpold, G H Rhodes, P L Bloch, D A Carson, J H Vaughan
The Journal of Immunology January 15, 1987, 138 (2) 593-599;

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The glycine-alanine repeating region is the major epitope of the Epstein-Barr nuclear antigen-1 (EBNA-1).
H Rumpold, G H Rhodes, P L Bloch, D A Carson, J H Vaughan
The Journal of Immunology January 15, 1987, 138 (2) 593-599;
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Print ISSN 0022-1767        Online ISSN 1550-6606