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Role of membrane potential in the response of human T lymphocytes to phytohemagglutinin.

E W Gelfand, R K Cheung, G B Mills and S Grinstein
J Immunol January 15, 1987, 138 (2) 527-531;
E W Gelfand
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R K Cheung
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G B Mills
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S Grinstein
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Abstract

We have analyzed the role of membrane potential on T cell activation and cell proliferation. Depolarization of T lymphocytes, by increasing the extracellular concentration of K+ during a 1-hr exposure to PHA, results in a marked inhibition of cell proliferation. In parallel, depolarization of T cells prevented the normal increase in [Ca2+]i seen after PHA binding. In depolarized cells, PHA failed to induce IL 2 secretion, but, in contrast, IL 2 receptor expression was triggered normally and the cells were subsequently responsive to exogenous IL 2. Increasing [Ca2+]i in depolarized cells with the ionophore ionomycin, or bypassing the requirement for an increase in [Ca2+]i with TPA, restored the PHA-induced proliferative response in depolarized cells. These data confirm that a membrane potential-sensitive step, namely, Ca2+ influx and the resulting change in [Ca2+]i, is triggered by PHA. The inhibitory effects of depolarization are mediated through the impairment of IL 2 secretion, but not IL 2 receptor expression. T cell proliferation can therefore be regulated by altering membrane potential, which in turn modulates the extent of the change in [Ca2+]i. This study suggests a role for transmembrane potential in the regulation of the T cell proliferative response.

  • Copyright © 1987 by American Association of Immunologists

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The Journal of Immunology
Vol. 138, Issue 2
15 Jan 1987
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Role of membrane potential in the response of human T lymphocytes to phytohemagglutinin.
E W Gelfand, R K Cheung, G B Mills, S Grinstein
The Journal of Immunology January 15, 1987, 138 (2) 527-531;

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Role of membrane potential in the response of human T lymphocytes to phytohemagglutinin.
E W Gelfand, R K Cheung, G B Mills, S Grinstein
The Journal of Immunology January 15, 1987, 138 (2) 527-531;
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Print ISSN 0022-1767        Online ISSN 1550-6606