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Isolation and analysis of primitive hemopoietic progenitor cells on the basis of differential expression of Qa-m7 antigen.

I Bertoncello, S H Bartelmez, T R Bradley, E R Stanley, R A Harris, M S Sandrin, A B Kriegler, I K McNiece, S D Hunter and G S Hodgson
J Immunol May 1, 1986, 136 (9) 3219-3224;
I Bertoncello
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S H Bartelmez
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T R Bradley
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E R Stanley
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R A Harris
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M S Sandrin
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A B Kriegler
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I K McNiece
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S D Hunter
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G S Hodgson
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Abstract

In the presence of the hemopoietic growth factor CSF-1, the later committed cells of the macrophage lineage can be detected by their ability to form small colonies in clonal agar culture (CFCCSF-1). Synergistic factors have been described that in combination with CSF-1 stimulate developmentally early hemopoietic progenitor cells of high proliferative potential (HPP-CFC). By using a monoclonal antibody to the Qa-m7 antigenic determinant, we investigated and compared the expression of Qa-m7 on CFCCSF-1 and on HPP-CFC of two types that grow in response to either 1) CSF-1 plus synergistic factor from human placenta-conditioned medium (HPP-CFCHplac+CSF-1) or 2) CSF-1 plus synergistic factor from conditioned medium of the WEHI-3 myelomonocytic cell line (HPP-CFCW+CSF-1). We have shown that HPP-CFC of both types express relatively more Qa-m7 antigen than CFCCSF-1 and can be separated and enriched on this basis by discontinuous buoyant density centrifugation and fluorescence-activated cell sorting of normal bone marrow. Significant enrichments of HPP-CFCHPlac+CSF-1 (43.5-fold) and HPP-CFCW+CSF-1 (28.8-fold) have been achieved with cloning efficiencies of HPP-CFC in the most enriched fractions reaching 4 to 5%. These results clearly illustrate the fact that there are populations of progenitor cells from normal, unperturbed bone marrow that strictly require a combination of two hemopoietic growth factors (CSF-1 plus synergistic factor) in order to be detected.

  • Copyright © 1986 by American Association of Immunologists

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The Journal of Immunology
Vol. 136, Issue 9
1 May 1986
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Isolation and analysis of primitive hemopoietic progenitor cells on the basis of differential expression of Qa-m7 antigen.
I Bertoncello, S H Bartelmez, T R Bradley, E R Stanley, R A Harris, M S Sandrin, A B Kriegler, I K McNiece, S D Hunter, G S Hodgson
The Journal of Immunology May 1, 1986, 136 (9) 3219-3224;

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Isolation and analysis of primitive hemopoietic progenitor cells on the basis of differential expression of Qa-m7 antigen.
I Bertoncello, S H Bartelmez, T R Bradley, E R Stanley, R A Harris, M S Sandrin, A B Kriegler, I K McNiece, S D Hunter, G S Hodgson
The Journal of Immunology May 1, 1986, 136 (9) 3219-3224;
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Print ISSN 0022-1767        Online ISSN 1550-6606