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Characterization of cell lines expressing mutant I-Ab and I-Ak molecules allows the definition of distinct serologic epitopes on A alpha and A beta polypeptides.

B N Beck, J M Buerstedde, C J Krco, A E Nilson, C G Chase and D J McKean
J Immunol April 15, 1986, 136 (8) 2953-2961;
B N Beck
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J M Buerstedde
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C J Krco
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A E Nilson
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C G Chase
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D J McKean
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Abstract

A series of seven I-Ab-reactive monoclonal antibodies (mAb) has been derived from a BALB/c anti-C57BL/6 immunization. Analysis of the reactivity patterns of these mAb with spleen cells of mice from the independent haplotypes has revealed three groups of mAb: group I mAb react with all haplotypes except d, group II with all except d and k, and group III with all except d, k, and j. In addition, the group I and group II mAb also react with human class II-expressing cells. We have used these mAb to isolate one mutant I-Ab-expressing cell line and three additional I-Ak mutant cell lines. These antibodies have been used, in conjunction with a large panel of I-A-reactive mAb available from others, to extensively characterize our collection of mutant I-A-expressing cell lines. Analysis of the mutant cell lines has allowed us to assign the reactivity of each mAb to either the A alpha- or the A beta-polypeptide. All seven newly isolated mAb appear to react with determinants on the A alpha-polypeptide. Furthermore, analysis of the panel of A alpha k- and A beta k-mutants has allowed us to discriminate at least five epitopes that are separable by mutation on the A beta k-polypeptide, and two epitopes on the A alpha k-polypeptide.

  • Copyright © 1986 by American Association of Immunologists

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The Journal of Immunology
Vol. 136, Issue 8
15 Apr 1986
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Characterization of cell lines expressing mutant I-Ab and I-Ak molecules allows the definition of distinct serologic epitopes on A alpha and A beta polypeptides.
B N Beck, J M Buerstedde, C J Krco, A E Nilson, C G Chase, D J McKean
The Journal of Immunology April 15, 1986, 136 (8) 2953-2961;

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Characterization of cell lines expressing mutant I-Ab and I-Ak molecules allows the definition of distinct serologic epitopes on A alpha and A beta polypeptides.
B N Beck, J M Buerstedde, C J Krco, A E Nilson, C G Chase, D J McKean
The Journal of Immunology April 15, 1986, 136 (8) 2953-2961;
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Print ISSN 0022-1767        Online ISSN 1550-6606