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Identification of a macrophage antigen-processing event required for I-region-restricted antigen presentation to T lymphocytes.

K Ziegler and E R Unanue
J Immunol November 1, 1981, 127 (5) 1869-1875;
K Ziegler
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E R Unanue
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Abstract

The mechanism of macrophage-antigen handling was studied using a system that involves the quantitation of the antigen-specific binding of Listeria monocytogenes-immune T cells to macrophages. Specific T cells did not bind to native antigen. Because the specific binding of T cells to macrophages could be measured during a short (5- to 15-min) interaction, it was possible to follow the temporal development of a T cell-binding substrate with increasing time of antigen-macrophage interaction. In contrast to the rapid (5-min) uptake of Listeria by macrophages, the development of T cell-binding ability required a 30- and 60-min period of antigen-macrophage interaction. During this processing period, Listeria organisms bound to the macrophage surface were ingested and partially catabolized. Unlike antigen uptake, antigen processing was a temperature-dependent and energy-requiring event. Although macrophages treated with paraformaldehyde before antigen processing did not develop T cell-binding activity, macrophages treated with paraformaldehyde after a 60-min antigen-processing period retained T cell-binding ability. The kinetics of antigen catabolism correlated with antigen processing, and inhibition of antigen catabolism was associated with a corresponding inhibition of antigen processing for T cell binding. Anti-Ia antibodies had no effect on Listeria uptake of catabolism. These results supply direct evidence for a macrophage-antigen processing event relevant to T cell recognition of antigen.

  • Copyright © 1981 by American Association of Immunologists

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The Journal of Immunology
Vol. 127, Issue 5
1 Nov 1981
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Identification of a macrophage antigen-processing event required for I-region-restricted antigen presentation to T lymphocytes.
K Ziegler, E R Unanue
The Journal of Immunology November 1, 1981, 127 (5) 1869-1875;

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Identification of a macrophage antigen-processing event required for I-region-restricted antigen presentation to T lymphocytes.
K Ziegler, E R Unanue
The Journal of Immunology November 1, 1981, 127 (5) 1869-1875;
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Print ISSN 0022-1767        Online ISSN 1550-6606