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Functional Characterization of Mouse Lymphocyte Subpopulations Identified by Their Natural Binding of Bacteria

I. Identification of the Ig-Secreting Cell Subpopulation

Wen-Yuan Chen, Marius Teodorescu and Eugene P. Mayer
J Immunol December 1, 1979, 123 (6) 2817-2822;
Wen-Yuan Chen
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Marius Teodorescu
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Eugene P. Mayer
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Abstract

Three mouse B cell subpopulations (B1, B2 and B3) can be identified by their natural binding of bacteria. To determine whether these subpopulations have unique functions, we assayed the number of anti-SRBC-secreting cells and the number of Ig-secreting cells in unseparated populations and in populations in which the B2 and B3 cells were removed by immobilized monolayers of Escherichia coli-2, a bacteria that binds B2 and B3 cells. Essentially all of the plaque-forming cells present in the unseparated population were found in the B1-enriched population, suggesting that most of the antibody-secreting and Ig-secreting cells were in the B1 subpopulation. To show conclusively that the anti-SRBC-secreting cells resided in the B1 subpopulation, the Jerne plaque assay was performed on slides by using lymphocytes prelabeled with various bacteria and the cells that gave rise to the plaques were directly examined. Essentially all of the secreting cells were labeled with Corynebacterium xerosis, which binds to the B1 and B2 cells, whereas very few of the secreting cells were labeled with Arizona hinshawii, which binds to the B2 cells, or with Escherichia coli-2, which binds to the B2 and B3 cells. Thus, the B1 subpopulation contained essentially all of the antibody-secreting cells, which indicates that the B cell subpopulations identified by bacteria are functionally different.

Footnotes

  • ↵2 In partial fullfillment of the requirements for the Doctor of Philosophy degree in the Graduate College, University of Illinois at the Medical Center, Chicago, Illinois.

  • ↵3 To whom correspondence and reprint requests should be addressed.

  • ↵1 This work was supported by National Institute of Allergy and Infectious Disease Grant 1RO1 AI 14630, National Institutes of Health Grant 2R01 AM 19414, and American Cancer Society Grant CH135.

  • Received June 8, 1979.
  • Accepted September 12, 1979.
  • Copyright © 1979 by The American Association of Immunologists, Inc.

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The Journal of Immunology
Vol. 123, Issue 6
1 Dec 1979
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Functional Characterization of Mouse Lymphocyte Subpopulations Identified by Their Natural Binding of Bacteria
Wen-Yuan Chen, Marius Teodorescu, Eugene P. Mayer
The Journal of Immunology December 1, 1979, 123 (6) 2817-2822;

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Functional Characterization of Mouse Lymphocyte Subpopulations Identified by Their Natural Binding of Bacteria
Wen-Yuan Chen, Marius Teodorescu, Eugene P. Mayer
The Journal of Immunology December 1, 1979, 123 (6) 2817-2822;
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Print ISSN 0022-1767        Online ISSN 1550-6606