Skip to main content

Main menu

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons

User menu

  • Subscribe
  • Log in

Search

  • Advanced search
The Journal of Immunology
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons
  • Subscribe
  • Log in
The Journal of Immunology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Follow The Journal of Immunology on Twitter
  • Follow The Journal of Immunology on RSS

The Requirement of Ia-Positive Accessory Cells for the Induction of Hapten-Reactive Cytotoxic T Lymphocytes in vitro

Uki Yamashita and Toshiyuki Hamaoka
J Immunol December 1, 1979, 123 (6) 2637-2643;
Uki Yamashita
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Toshiyuki Hamaoka
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

The antigen-presenting mechanism to hapten-reactive cytotoxic T cells was studied by using fractionated responder T cells, hapten-modified syngeneic stimulator cells, and accessory cells. When the effect of various treatments on the stimulatory activity by spleen cells in the induction of cytotoxic T cells was studied by coculturing TNP-modified spleen cells and nylon wool column-purified T cells, the stimulatory activity was associated with the spleen cell population adhering to plastic dishes, Sephadex G-10, and nylon wool, and was sensitive to anti-Ia serum and complement treatment. The activity was resistant to anti-Th B serum and anti-Thy 1 serum and complement treatment. In order to figure out the role of Ia antigens for induction of cytotoxic T cells, Ia-negative tumor cells were used as stimulator cells with TNP-modification, and the effect of addition of spleen cells containing Ia-positive cells to the system was studied. The addition of accessory cells restored the induction of TNP-reactive cytotoxic T cell activity under conditions in which the TNP-modified tumor cells alone could not induce cytotoxic T cells to stimulate nylon wool column-purified T cells. In this system, not only syngeneic but also allogeneic spleen cells had the accessory activity. Anti-Ia serum could inhibit the induction of cytotoxic T cells when added continuously to the culture, and this inhibition was specific for the accessory cells added, but not for the responder T cells or stimulator cells. Thus, Ia-positive accessory cells seem to play a crucial role in the induction of cytotoxic T cells in vitro. The possible role of Ia-positive accessory cells is discussed in the context of cellular interactions in the antigen-recognition mechanism of cytotoxic T cells.

Footnotes

  • ↵1 This work was supported by a Grant-in-Aid for Scientific Research from the Japanese Government.

  • Received June 26, 1979.
  • Accepted September 4, 1979.
  • Copyright © 1979 by The American Association of Immunologists, Inc.

Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$37.50

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

Log in using your username and password

Forgot your user name or password?
PreviousNext
Back to top

In this issue

The Journal of Immunology
Vol. 123, Issue 6
1 Dec 1979
  • Table of Contents
  • Table of Contents (PDF)
  • Advertising (PDF)
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about The Journal of Immunology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
The Requirement of Ia-Positive Accessory Cells for the Induction of Hapten-Reactive Cytotoxic T Lymphocytes in vitro
(Your Name) has forwarded a page to you from The Journal of Immunology
(Your Name) thought you would like to see this page from the The Journal of Immunology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
The Requirement of Ia-Positive Accessory Cells for the Induction of Hapten-Reactive Cytotoxic T Lymphocytes in vitro
Uki Yamashita, Toshiyuki Hamaoka
The Journal of Immunology December 1, 1979, 123 (6) 2637-2643;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
The Requirement of Ia-Positive Accessory Cells for the Induction of Hapten-Reactive Cytotoxic T Lymphocytes in vitro
Uki Yamashita, Toshiyuki Hamaoka
The Journal of Immunology December 1, 1979, 123 (6) 2637-2643;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like

Jump to section

  • Article
  • Info & Metrics
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Antigen Specific T Cell-Mediated Suppression
  • Cellular and Genetic Control of Antibody Responses
  • The Qa-2 Antigen on Lymphocyte Subpopulations. Mixed Lymphocyte Culture and Cell-Mediated Lympholysis
Show more Immunogenetics and Transplantation

Similar Articles

Navigate

  • Home
  • Current Issue
  • Next in The JI
  • Archive
  • Brief Reviews
  • Pillars of Immunology
  • Translating Immunology

For Authors

  • Submit a Manuscript
  • Instructions for Authors
  • About the Journal
  • Journal Policies
  • Editors

General Information

  • Advertisers
  • Subscribers
  • Rights and Permissions
  • Accessibility Statement
  • Public Access
  • Privacy Policy
  • Disclaimer

Journal Services

  • Email Alerts
  • RSS Feeds
  • ImmunoCasts
  • Twitter

Copyright © 2021 by The American Association of Immunologists, Inc.

Print ISSN 0022-1767        Online ISSN 1550-6606