Abstract
Chemically induced tumors of mice exhibit apparently unique antigenicity upon syngeneic transplantation into appropriately immunized hosts. An in vitro counterpart of this pattern in terms of specificity has not been reported. Data are presented that demonstrate that immune peritoneal exudates contain cells cytotoxic for the specific immunogen tumor but with rare exceptions, not toward other syngeneic methylcholanthrene-induced fibrosarcomas. Only tumors highly immunogenic by transplantation criteria induce cytotoxic PEC regularly; nonimmunogenic tumors consistently fail to do so. The effector cell responsible is eliminated by pretreatment with anti-Thy.1 but not anti-Ig plus complement. In concomitant experiments, PEC populations cytotoxic in vitro also conveyed adoptive protection against the specific tumor in syngeneic hosts. This in vitro assay appears to provide a tool for studying T cell-mediated cytotoxicity toward a set of unique surface antigens present on chemically induced tumors.
Footnotes
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↵1 This work was supported in part by National Institutes of Health Grants HD-00384, CA-15334, and gifts to the Mary and Ryan Whisenant Cancer Research Fund. Dr. Chauvenet was a postdoctoral fellow in Tumor Biology supported by The National Cancer Institute Training Program, CA-09126. Dr. Smith was a Josiah Macy Foundation Scholar in Medical Sciences at The Jackson Laboratory, Bar Harbor, Maine, and The Basel Institute, Basel, Switzerland. This is Tumor Biology Unit Publication No. 145 from the Tumor Biology Unit, Department of Pathology, University of Florida College of Medicine, Gainesville, Florida 32610.
- Received January 23, 1979.
- Accepted August 23, 1979.
- Copyright © 1979 by The American Association of Immunologists, Inc.
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