Abstract
Antigen-induced proliferation of primed lymph node cells was abrogated by treatment with anti-Ly 1 serum and complement (C) but not with anti-Ly 2 serum and C. Lymph node cells from animals primed to ovalbumin were activated with antigen in vitro, followed by propagation in an antigen-free supernatant fluid obtained from lectin-induced normal spleen cells. T cells processed in this manner displayed a stepwise enrichment of helper activity for antibody production as measured in a secondary hapten-carrier response. The sequential increase in antigen-specific help was paralleled by a rise in the antigen-induced proliferative response, a phenomenon whose expression was dependent on the presence of syngeneic or semi-syngeneic irradiated filler cells.
Footnotes
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↵2 Supported by Training Grant IT32, AI 07035.
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↵3 Supported by National Institutes of Health Service Research Award IF 32AI 05479.
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↵4 Supported by National Institutes of Health Postdoctoral Fellowship F32 AI 05545.
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↵5 Supported by United States Public Health Service Research Career Development Award AI 00133.
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↵1 This work was supported by United States Public Health Service Grants AI 13131 and CA 21825.
- Received July 6, 1979.
- Accepted August 22, 1979.
- Copyright © 1979 by The American Association of Immunologists, Inc.
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