Abstract
We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies. Screening of hybrid cell lines for specificity and immunoglobulin classes was done with a modified enzyme-linked immunosorbent assay.
Footnotes
-
↵2 Address correspondence to Dr. J. Kearney, 224 Tumor Institute, University Station, University of Alabama in Birmingham, Birmingham, Alabama 35294.
-
↵1 This work was supported by the Deutsche Forschungsgemeinschaft through Sonderforschungsbereich 74 and Grants CA 16673 and A 114782. J. K. was supported by an EMBO senior fellowship and is a recipient of RCDA award 1 K04 AI/CA 00338.
- Received April 30, 1979.
- Accepted July 3, 1979.
- Copyright © 1979 by The American Association of Immunologists, Inc.
In this issue
