Skip to main content

Main menu

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons

User menu

  • Subscribe
  • Log in

Search

  • Advanced search
The Journal of Immunology
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons
  • Subscribe
  • Log in
The Journal of Immunology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Follow The Journal of Immunology on Twitter
  • Follow The Journal of Immunology on RSS

IgD Is Present on the Cell Surface of Murine Lymphocytes in Two Forms: δ2L2 and δL

Leon Eidels
J Immunol August 1, 1979, 123 (2) 896-902;
Leon Eidels
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

Murine cell surface immunoglobulin (Ig) isotypes were analyzed by lactoperoxidase-catalyzed radioiodination, immunoprecipitation, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE analysis revealed three radioactive peaks: I) IgM (µ2L2; 224,000 daltons), II) IgD (δ2L2; 185,000 daltons), and III) IgD half-molecules (δL; 105,000 daltons). The size of the δ-chain obtained from both the δ2L2 and the δL molecules was the same (68,000 daltons). The δL molecules are apparently not the result of a sulfhydryl-catalyzed reduction or disulfide interchange and are probably not generated by proteolysis, since addition of alkylating agents and protease inhibitors did not decrease the amount of δL observed. Addition of sulfhydryl-oxidizing agents also did not decrease the amount of δL, suggesting that the δL molecules cannot be reoxidized to yield δ2L2 molecules. The percentage of IgD in the δL form present on lymph node and spleen cells of A/J mice and on spleen cells of BALB/c mice was the same (∼55%). The percentage of δL on splenocytes from BDF/1 mice (2 to 7 months old) was ∼42%. Spleen cells from strains that are “IgD-deficient,” CBA/N and NZB, and spleen cells from 2-week-old BDF/1 mice, which have a low δ/µ ratio, displayed a coordinate decrease in the levels of both δ2L2 and δL. This coordinate decrease resulted in no alternation in the percentage of IgD present in the δL form (∼42%). These results, taken together, indicate that indeed the cell surface Ig of murine lymphoid cell populations consists of monomeric IgM, monomeric IgD, and IgD half-molecules.

Footnotes

  • ↵1 This work was supported by United States Public Health Service Grant AI 11851.

  • Received March 30, 1979.
  • Accepted May 21, 1979.
  • Copyright © 1979 by The American Association of Immunologists, Inc.

Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$37.50

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

Log in using your username and password

Forgot your user name or password?
PreviousNext
Back to top

In this issue

The Journal of Immunology
Vol. 123, Issue 2
1 Aug 1979
  • Table of Contents
  • Table of Contents (PDF)
  • Advertising (PDF)
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about The Journal of Immunology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
IgD Is Present on the Cell Surface of Murine Lymphocytes in Two Forms: δ2L2 and δL
(Your Name) has forwarded a page to you from The Journal of Immunology
(Your Name) thought you would like to see this page from the The Journal of Immunology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
IgD Is Present on the Cell Surface of Murine Lymphocytes in Two Forms: δ2L2 and δL
Leon Eidels
The Journal of Immunology August 1, 1979, 123 (2) 896-902;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
IgD Is Present on the Cell Surface of Murine Lymphocytes in Two Forms: δ2L2 and δL
Leon Eidels
The Journal of Immunology August 1, 1979, 123 (2) 896-902;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like

Jump to section

  • Article
  • Info & Metrics
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Sex-Related Immunocompetence of BALB/c Mice
  • Regulation of Antibody Response in Different Immunoglobulin Classes
  • Generation of Biologic Activity from the Purified α-Chain of C5
Show more Immunochemistry

Similar Articles

Navigate

  • Home
  • Current Issue
  • Next in The JI
  • Archive
  • Brief Reviews
  • Pillars of Immunology
  • Translating Immunology

For Authors

  • Submit a Manuscript
  • Instructions for Authors
  • About the Journal
  • Journal Policies
  • Editors

General Information

  • Advertisers
  • Subscribers
  • Rights and Permissions
  • Accessibility Statement
  • FAR 889
  • Privacy Policy
  • Disclaimer

Journal Services

  • Email Alerts
  • RSS Feeds
  • ImmunoCasts
  • Twitter

Copyright © 2022 by The American Association of Immunologists, Inc.

Print ISSN 0022-1767        Online ISSN 1550-6606