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Regulation of the Primary Immune Response to Ovalbumin in Mice: Activation of T Cells Mediating Delayed-Type Hypersensitivity, Nonspecific Help, and Specific Help, and Their Sensitivity to Radiation

Martha Turner Lubet and John R. Kettman
J Immunol July 1, 1979, 123 (1) 426-433;
Martha Turner Lubet
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John R. Kettman
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Abstract

We have previously shown that the immune response to ovalbumin (OVA) in mice is controlled by at least four genes. The purpose of the studies reported here was to investigate the site of action of the genes regulating the response to OVA. To this end, helper T cell functions were studied in responder (BDF1) and nonresponder (A/J) mice by using three approaches.

In the first approach, the antibody response to limiting and nonlimiting doses of TNP-OVA was studied. When mice were immunized with limiting doses of TNP-OVA, anti-TNP antibodies were elicited but anti-OVA antibodies were not. These findings suggest that OVA-specific helper T cells are not the limiting factor in the anti-OVA antibody responses.

Helper T cell functions were also measured in in vitro assays. Two kinds of helper T cell activity were measured. “Nonspecific” helper T cell activity (the bystander effect) was determined by measuring the ability of OVA-primed spleen cells to help the anti-sheep red blood cells (SRBC) antibody response in cultures stimulated with SRBC and TNP-OVA. Antigen-specific helper activity was determined by measuring the ability of OVA-primed spleen cells to help the anti-TNP antibody response in cultures stimulated with TNP-OVA. The results indicate that in vivo-priming doses of OVA that do not elicit a delayed-type hypersensitivity (DTH) response or a primary antibody response prime for both “nonspecific” and antigen-specific helper T cells. These findings suggest that the genes regulating the immune response to OVA do not control activation of helper T cells.

These T cell subpopulations that recognize OVA can be further distinguished by virtue of their sensitivity to radiation. The DTH effector cells and cells responsible for “nonspecific” help are not sensitive to 1000R. In contrast, the specific helper activity is sensitive to 1000R. The subsets that are not sensitive to radiation are distinguished by their independent activation by OVA.

Footnotes

  • ↵2 M. T. L. was a recipient of the American Association of University Women's Ida M. Green predoctoral fellowship.

  • ↵1 Supported by National Institutes of Health Grants AI 11630 and AI 11851.

  • Received February 14, 1979.
  • Accepted April 24, 1979.
  • Copyright, 1979, by The Williams & Wilkins Company
  • Copyright © 1979 by The American Association of Immunologists, Inc.

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The Journal of Immunology
Vol. 123, Issue 1
1 Jul 1979
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Regulation of the Primary Immune Response to Ovalbumin in Mice: Activation of T Cells Mediating Delayed-Type Hypersensitivity, Nonspecific Help, and Specific Help, and Their Sensitivity to Radiation
Martha Turner Lubet, John R. Kettman
The Journal of Immunology July 1, 1979, 123 (1) 426-433;

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Regulation of the Primary Immune Response to Ovalbumin in Mice: Activation of T Cells Mediating Delayed-Type Hypersensitivity, Nonspecific Help, and Specific Help, and Their Sensitivity to Radiation
Martha Turner Lubet, John R. Kettman
The Journal of Immunology July 1, 1979, 123 (1) 426-433;
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Print ISSN 0022-1767        Online ISSN 1550-6606