Skip to main content

Main menu

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons

User menu

  • Subscribe
  • Log in

Search

  • Advanced search
The Journal of Immunology
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons
  • Subscribe
  • Log in
The Journal of Immunology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Follow The Journal of Immunology on Twitter
  • Follow The Journal of Immunology on RSS

The Suppression of Mitogen- and Alloantigen-Stimulated Peripheral Blood Lymphocytes by Cultured Human T Lymphocytes

Richard D. Maca, Guy D. Bonnard and Ronald B. Herberman
J Immunol July 1, 1979, 123 (1) 246-251;
Richard D. Maca
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Guy D. Bonnard
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ronald B. Herberman
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

Cultured T lymphocytes (CTC), which were grown in medium containing a growth-promoting factor from supernatants of pooled, allogeneic, PHA-stimulated PBL, were examined for their ability to suppress mitogen-induced proliferative responses or MLC reactions. This suppressor activity was assayed by adding the CTC to either Con A- or PHA-treated lymphocytes or to a mixed lymphocyte culture. The incorporation of 3HTdR into nuclear protein was then quantitated at the end of the culture period and compared to the control 3HTdR uptake values. The addition of CTC significantly suppressed thymidine incorporation in both the mitogen- and alloantigen-stimulated cultures. This suppressed thymidine uptake was not due to competitive inhibition by cold thymidine released into the culture medium by the CTC. Likewise, this suppressor activity could not be accounted for by a “crowding” effect of the added CTC or by the depletion of nutrients from the culture medium by metabolizing CTC. CTC autologous to the responding normal lymphocytes were as effective in suppressing the mitogen and alloantigen responses as allogeneic CTC, indicating that the observed suppression by CTC could not be explained by the cytotoxicity by alloantigen-sensitized or polyclonal-activated CTC. Viable and irradiated CTC were equally effective in suppressing the Con A response. However, in the response to allogeneic cells, irradiating the CTC completely abrogated the suppressor effect. This model system provides a good approach for studying suppressor activity of human T lymphocytes that are not contaminated by other types of cells with suppressor potential, such as monocytes. In addition, this system should provide a means to evaluate ways by which this T cell suppressor activity can be modulated.

Footnotes

  • ↵2 To whom requests for reprints should be sent.

  • Received August 26, 1978.
  • Accepted April 13, 1979.
  • Copyright, 1979, by The Williams & Wilkins Company
  • Copyright © 1979 by The American Association of Immunologists, Inc.

Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$37.50

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

Log in using your username and password

Forgot your user name or password?
PreviousNext
Back to top

In this issue

The Journal of Immunology
Vol. 123, Issue 1
1 Jul 1979
  • Table of Contents
  • Table of Contents (PDF)
  • Advertising (PDF)
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about The Journal of Immunology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
The Suppression of Mitogen- and Alloantigen-Stimulated Peripheral Blood Lymphocytes by Cultured Human T Lymphocytes
(Your Name) has forwarded a page to you from The Journal of Immunology
(Your Name) thought you would like to see this page from the The Journal of Immunology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
The Suppression of Mitogen- and Alloantigen-Stimulated Peripheral Blood Lymphocytes by Cultured Human T Lymphocytes
Richard D. Maca, Guy D. Bonnard, Ronald B. Herberman
The Journal of Immunology July 1, 1979, 123 (1) 246-251;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
The Suppression of Mitogen- and Alloantigen-Stimulated Peripheral Blood Lymphocytes by Cultured Human T Lymphocytes
Richard D. Maca, Guy D. Bonnard, Ronald B. Herberman
The Journal of Immunology July 1, 1979, 123 (1) 246-251;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like

Jump to section

  • Article
  • Info & Metrics
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Differential Susceptibility to Staphylococcal Superantigen (SsAg)-Induced Apoptosis of CD4+ T Cells from Atopic Dermatitis Patients and Healthy Subjects: The Inhibitory Effect of IL-4 on SsAg-Induced Apoptosis
  • HIV-1 Vaccination Administered Intramuscularly Can Induce Both Systemic and Mucosal T Cell Immunity in HIV-1-Uninfected Individuals
  • Osteopontin (Eta-1) and Fibroblast Growth Factor-2 Cross-Talk in Angiogenesis
Show more Clinical Immunology

Similar Articles

Navigate

  • Home
  • Current Issue
  • Next in The JI
  • Archive
  • Brief Reviews
  • Pillars of Immunology
  • Translating Immunology

For Authors

  • Submit a Manuscript
  • Instructions for Authors
  • About the Journal
  • Journal Policies
  • Editors

General Information

  • Advertisers
  • Subscribers
  • Rights and Permissions
  • Accessibility Statement
  • Public Access
  • Privacy Policy
  • Disclaimer

Journal Services

  • Email Alerts
  • RSS Feeds
  • ImmunoCasts
  • Twitter

Copyright © 2021 by The American Association of Immunologists, Inc.

Print ISSN 0022-1767        Online ISSN 1550-6606